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Genetic alterations, such as loss of heterozygosity (LOH) or microsatellite instability (MI), have been reported in both malignant and benign disorders. In order to identify loci of deoxyribonucleic acid (DNA) mutation in asthma, MI and LOH were studied in sputum cells. DNA was extracted from cells in the sputum and blood cells of 22 patients with moderate asthma. Cells were analysed for MI and LOH using 18 polymorphic markers on chromosome 5q, 6p, 11q, 14q. Microsatellite analysis was also performed in six healthy subjects. None of the healthy individuals exhibited any genetic alteration. Genetic alterations were found in 16 of 22 asthmatic patients (73%). In total, 12 (54.5%) patients exhibited LOH only, one (4.5%) MI only, while three showed both MI and LOH. The highest incidence of LOH and MI was found on chromosome 14q. Mean immunoglobulin E and blood eosinophil levels were significantly higher in asthmatics with three or more genetic alterations. A high incidence of genetic alterations in the deoxyribonucleic acid of the sputum cells was found in asthmatic patients. Further studies are needed to identify the role of loss of heterozygosity and microsatellite instability in the investigation of genetic susceptibility of asthma and thus, in its pathogenesis.
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
November 1999,
American journal of respiratory and critical care medicine,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
October 1995,
The Journal of investigative dermatology,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
January 2011,
Medicina (Kaunas, Lithuania),
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
December 1997,
Laboratory investigation; a journal of technical methods and pathology,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
June 1997,
The British journal of ophthalmology,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
April 1994,
Cancer research,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
February 1997,
International journal of oncology,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
March 1997,
Archives of dermatological research,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
January 1997,
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
E Paraskakis, and
G Sourvinos, and
F Passam, and
N Tzanakis, and
E G Tzortzaki, and
M Zervou, and
D Spandidos, and
N M Siafakas
June 2000,
Journal of pediatric surgery,
