Biomaterial Database

Time correction for computing Michaelis--Menten kinetics. 1992

F Rodriguez, and M Prats

Centre de Recherches de Biochimie et de Génétique Cellulaires, Toulouse, France.

A description of time correction applied to the integrated Michaelis equation in view of cancelling a non-correct estimation of the Michaelis constant due to a lack of coincidence between time zero of measurement and time zero of reaction is presented. An estimation of kinetic parameters is made with time correction on the same experimental set of data using a classical least squares iterative procedure.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008838	Microcomputers	Small computers using LSI (large-scale integration) microprocessor chips as the CPU (central processing unit) and semiconductor memories for compact, inexpensive storage of program instructions and data. They are smaller and less expensive than minicomputers and are usually built into a dedicated system where they are optimized for a particular application. "Microprocessor" may refer to just the CPU or the entire microcomputer.	Computers, Personal,Microprocessors,Computer, Personal,Microcomputer,Microprocessor,Personal Computer,Personal Computers
D004798	Enzymes	Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.	Biocatalyst,Enzyme,Biocatalysts
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D015219	Substrate Cycling	A set of opposing, nonequilibrium reactions catalyzed by different enzymes which act simultaneously, with at least one of the reactions driven by ATP hydrolysis. The results of the cycle are that ATP energy is depleted, heat is produced and no net substrate-to-product conversion is achieved. Examples of substrate cycling are cycling of gluconeogenesis and glycolysis pathways and cycling of the triglycerides and fatty acid pathways. Rates of substrate cycling may be increased many-fold in association with hypermetabolic states resulting from severe burns, cold exposure, hyperthyroidism, or acute exercise.	Futile Cycling,Futile Cycles,Futile Substrate Cycles,Futile Substrate Cycling,Cycle, Futile,Cycle, Futile Substrate,Cycles, Futile,Cycles, Futile Substrate,Cycling, Futile,Cycling, Futile Substrate,Cycling, Substrate,Cyclings, Futile,Cyclings, Futile Substrate,Cyclings, Substrate,Futile Cycle,Futile Cyclings,Futile Substrate Cycle,Futile Substrate Cyclings,Substrate Cycle, Futile,Substrate Cycles, Futile,Substrate Cycling, Futile,Substrate Cyclings,Substrate Cyclings, Futile
D016018	Least-Squares Analysis	A principle of estimation in which the estimates of a set of parameters in a statistical model are those quantities minimizing the sum of squared differences between the observed values of a dependent variable and the values predicted by the model.	Rietveld Refinement,Analysis, Least-Squares,Least Squares,Analyses, Least-Squares,Analysis, Least Squares,Least Squares Analysis,Least-Squares Analyses,Refinement, Rietveld