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An endoglucanase I (EG1) from a fungal source (Humicola insolens) has been crystallized in a number of forms suitable for X-ray diffraction analysis. Four crystal forms have been grown from various precipitants using vapour phase diffusion methods in hanging drops. Three of these crystal forms diffract to beyond 2.5 A resolution. Two forms, obtained from ammonium sulphate at pH 5.4, or 8.0, grow as tetragonal bipyramids in space group P4(1)22 or P4(3)22, with approximate cell dimensions a = b = 102 A, c = 282 A. The other crystal forms were grown from polyethylene glycol 8000 at pH 8.0. One grows as monoclinic plates, space group P2(1), with cell dimensions a = 66.9 A, b = 75.2 A, c = 86.9 A and beta = 102.9 degrees and the other as long hexagonal rods in space group P6(1)22 or P6(5)22, with cell dimensions a = b = 119 A, c = 83 A.
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
December 2008,
Acta crystallographica. Section F, Structural biology and crystallization communications,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
January 2003,
Acta crystallographica. Section D, Biological crystallography,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
March 2012,
Acta crystallographica. Section F, Structural biology and crystallization communications,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
September 1997,
Acta crystallographica. Section D, Biological crystallography,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
March 2012,
Acta crystallographica. Section F, Structural biology and crystallization communications,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
August 2003,
Acta crystallographica. Section D, Biological crystallography,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
September 1993,
Journal of molecular biology,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
December 1993,
Journal of molecular biology,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
July 2004,
Acta crystallographica. Section D, Biological crystallography,
G Davies, and
S Tolley, and
K Wilson, and
M Schülein, and
H F Wöldike, and
G Dodson
May 1986,
Journal of molecular biology,
