BIOMAT Database Logo BIOMAT Database Logo

Whole blood versus plasma spots for measurement of HIV-1 viral load in HIV-infected African patients. 2003

Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
University of Zambia-University College London Medical School (UNZA-UCLMS) Research and Training Project, University Teaching Hospital, Lusaka, Zambia. pbmwaba2000@yahoo.com

The increasing availability of antiretroviral drugs to HIV-infected populations in developing countries highlights the need to develop field-friendly practical methods for HIV-1 viral load measurements to monitor the effects of treatment. We compared use of whole-blood spots versus plasma dried on filter paper to quantify HIV-1 viral load in 51 African patients with HIV-1. The mean log10 HIV-1 viral loads were 4.22 for dried plasma spots (DPS) and 4.20 for dried whole-blood spots (DBS). The difference between the pairs of log10 viral load for DPS and DBS were significantly correlated with their mean (Spearman's r=0.31, p=0.03). This correlation between the difference and mean of viral load was no longer evident for values of log10 DPS that were less than 5 (r=0.01, p=0.93). For the 38 paired values with log10DPS of less than 5, the mean difference (log10DPS-log10DBS) was -0.04 (SD 0.29). Dried whole blood stored on filter paper at room temperature shows potential as a field-friendly alternative to plasma for measurement of HIV-1 viral load.

UI MeSH Term Description Entries
D010949 Plasma The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION. Blood Plasma,Fresh Frozen Plasma,Blood Plasmas,Fresh Frozen Plasmas,Frozen Plasma, Fresh,Frozen Plasmas, Fresh,Plasma, Blood,Plasma, Fresh Frozen,Plasmas,Plasmas, Blood,Plasmas, Fresh Frozen
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D001800 Blood Specimen Collection The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc. Blood Specimen Collections,Collection, Blood Specimen,Collections, Blood Specimen,Specimen Collection, Blood,Specimen Collections, Blood
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D014766 Viremia The presence of viruses in the blood. Viremias
D015024 Zambia A republic in southern Africa, south of DEMOCRATIC REPUBLIC OF THE CONGO and TANZANIA, and north of ZIMBABWE. Its capital is Lusaka. It was formerly called Northern Rhodesia. Northern Rhodesia,Rhodesia, Northern,Republic of Zambia
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D015658 HIV Infections Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS). HTLV-III Infections,HTLV-III-LAV Infections,T-Lymphotropic Virus Type III Infections, Human,HIV Coinfection,Coinfection, HIV,Coinfections, HIV,HIV Coinfections,HIV Infection,HTLV III Infections,HTLV III LAV Infections,HTLV-III Infection,HTLV-III-LAV Infection,Infection, HIV,Infection, HTLV-III,Infection, HTLV-III-LAV,Infections, HIV,Infections, HTLV-III,Infections, HTLV-III-LAV,T Lymphotropic Virus Type III Infections, Human

Related Publications

Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Immune activation and plasma viral load in HIV-infected African individuals.
July 1999, AIDS (London, England),
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Evaluation of non-centrifuged dried plasma spots versus centrifuged and non-centrifuged plasma for determination of HIV-1 viral load.
April 2013, Journal of virological methods,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Finger Prick Dried Blood Spots for HIV Viral Load Measurement in Field Conditions in Zimbabwe.
January 2015, PloS one,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Correlation between HIV-1 viral load quantification in plasma, dried blood spots, and dried plasma spots using the Roche COBAS Taqman assay.
January 2010, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Separation of Plasma from Whole Blood by Use of the cobas Plasma Separation Card: a Compelling Alternative to Dried Blood Spots for Quantification of HIV-1 Viral Load.
April 2019, Journal of clinical microbiology,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Use of Dried Plasma Spots for HIV-1 Viral Load Determination and Drug Resistance Genotyping in Mexican Patients.
January 2015, BioMed research international,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
RANTES production in HIV-1 antigen-stimulated whole blood culture: relationship with type 1 immune response and plasma viral load in individuals infected with HIV-1.
August 1998, Scandinavian journal of immunology,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Feasibility of using dried blood spots for HIV viral load testing among HIV-infected individuals in Thailand using QIAGEN QIAsymphony-artus HIV-1 platform.
September 2019, Journal of medical virology,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Plasma Epstein-Barr viral load measurement as a diagnostic marker of lymphoma in HIV-infected patients.
October 2010, Medicina clinica,
Peter Mwaba, and Sharon Cassol, and Andrew Nunn, and Rick Pilon, and Chifumbe Chintu, and Michelle Janes, and Alimuddin Zumla
Cellular and plasma viral load in patients infected with HIV-2.
November 1993, AIDS (London, England),
Copied contents to your clipboard!