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A lectin with a high affinity for glucose/mannose was isolated from Annona muricata seeds (Annonaceae) by gel filtration chromatography on Sephacryl S-200, ion exchange chromatography on a DEAE SP-5 PW column, and molecular exclusion on a Protein Pak Glass 300 SW column. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) yielded two protein bands of approximately 14 kDa and 22 kDa. However, only one band was seen in native PAGE. The Mr of the lectin estimated by fast-performance liquid chromatography-gel filtration on Superdex 75 was 22 kDa. The lectin was a glycoprotein with 8% carbohydrate (neutral sugar) and required divalent metal cations (Ca2+, Mg2+, and Mn2+) for full activity. Amino acid analysis revealed a large content of Glx, Gly, Phe, and Lys. The lectin agglutinated dog, chicken, horse, goose, and human erythrocytes and inhibited the growth of the fungi Fusarium oxysporum, Fusarium solani, and Colletotrichum musae.
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M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
April 2003,
Protein and peptide letters,
D C S Damico, and
M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
October 1991,
Planta medica,
D C S Damico, and
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M L R Macedo
January 2001,
Journal of Asian natural products research,
D C S Damico, and
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J C Novello, and
M L R Macedo
February 1996,
Journal of natural products,
D C S Damico, and
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S Marangoni, and
J C Novello, and
M L R Macedo
October 1983,
The Biochemical journal,
D C S Damico, and
M G M Freire, and
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M H Toyama, and
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J C Novello, and
M L R Macedo
January 1990,
Phytochemistry,
D C S Damico, and
M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
November 1998,
Phytochemistry,
D C S Damico, and
M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
June 1978,
Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
D C S Damico, and
M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
July 1998,
The international journal of biochemistry & cell biology,
D C S Damico, and
M G M Freire, and
V M Gomes, and
M H Toyama, and
S Marangoni, and
J C Novello, and
M L R Macedo
April 1998,
Phytochemistry,
