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The movement protein of cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro. 2004

C M Carvalho, and J Pouwels, and J W M van Lent, and T Bisseling, and R W Goldbach, and J Wellink
Laboratory of Virology, Department of Plant Sciences, Wageningen University, 6709 PD Wageningen, The Netherlands.

The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a non-sequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

UI MeSH Term Description Entries
D011523 Protoplasts The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL. Protoplast
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004277 DNA, Single-Stranded A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006160 Guanosine Triphosphate Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety. GTP,Triphosphate, Guanosine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D053830 Plant Viral Movement Proteins Viral proteins that facilitate the movement of viruses between plant cells by means of PLASMODESMATA, channels that traverse the plant cell walls. Movement Proteins, Viral, Plant,Viral Movement Proteins, Plant,Movement Protein, Plant Virus,Movement Proteins, Plant Virus,Movement Proteins, Virus, Plant,Plant Virus Movement Proteins,Virus Movement Proteins, Plant
D017798 Comovirus A genus of plant viruses of the family COMOVIRIDAE in which the bipartite genome is encapsidated in separate icosahedral particles. Mosaic and mottle symptoms are characteristic, and transmission is exclusively by leaf-feeding beetles. Cowpea mosaic virus is the type species. Cowpea Mosaic Virus,Cowpea Mosaic Viruses,Mosaic Virus, Cowpea,Mosaic Viruses, Cowpea

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