Direct activation of HSP90A transcription by c-Myc contributes to c-Myc-induced transformation. 2004

Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
Institute of Biochemistry, National Yang-Ming University, Taipei 112, Taiwan.

The c-myc proto-oncogene encodes a ubiquitous transcription factor involved in the control of cell growth and differentiation and implicated in inducing tumorigenesis. Understanding the function of c-Myc and its role in cancer depends upon the identification of c-Myc target genes. Heat shock protein 90 (HSP90) is involved in the folding of proteins such as signal transduction molecules (Src, Raf1, cdk4) and steroid receptors and in enhancing the activity of telomerase and nitric-oxide synthase. Here we show that c-Myc directly activates HSP90A transcription. c-Myc-mediated induction of HSP90A transcription occurs in different tissues, is independent of cell proliferation, and is mediated by a c-Myc binding site in the proximal promoter region of HSP90A gene. Overexpression of HSP90A in Rat1a cells induces transformation. Short interference RNA of HSP90A/Hsp86alpha reduces transformation activity in HeLa and RatMyc cells. These results indicate that by induction of HSP90A c-Myc may control the activity of multiple signal pathways involved in cellular transformation.

UI MeSH Term Description Entries
D008156 Luciferases Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates. Luciferase
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011233 Precipitin Tests Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate. Precipitin Test,Test, Precipitin,Tests, Precipitin
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002471 Cell Transformation, Neoplastic Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill. Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D002843 Chromatin The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. Chromatins
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning

Related Publications

Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
February 1999, Nature genetics,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
January 2003, Nature,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
May 2007, Oncogene,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
December 2007, Cancer research,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
April 2020, Oncogene,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
February 1993, Molecular and cellular biology,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
December 2008, FEBS letters,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
October 2003, The Journal of biological chemistry,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
August 1994, Cancer letters,
Shu-Chun Teng, and Yung-Yi Chen, and Yi-Ning Su, and Po-Chien Chou, and Yu-Chi Chiang, and Shun-Fu Tseng, and Kou-Juey Wu
February 1985, Japanese journal of cancer research : Gann,
Copied contents to your clipboard!