Cdc42-dependent nuclear translocation of non-receptor tyrosine kinase, ACK. 2004

Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854, USA.

Ras signals for the transformation of mammalian cells are apparently transduced through Rho GTPases. The Rho GTPase family member Cdc42 generates independent signals that regulate the rearrangement of the actin cytoskeleton and the transcription of genes. However, the molecular mechanism of signal transduction from Cdc42 to the nucleus remains to be understood. The non-receptor tyrosine kinases ACK-1 and ACK-2 have been found to bind specifically to Cdc42. In this paper we studied whether ACKs transduce Cdc42 signals to the nucleus directly, or through other cytoplasmic proteins. Using immunocytochemistry and Western blot analysis, we found a nuclear localization of ACKs in semi-confluent glioblastoma (U251) cells, as opposed to a cytosolic localization in confluent cells. In agreement with the nuclear localization, a putative nuclear export signal was identified in ACK-1 and ACK-2. Furthermore, the interaction of Cdc42 with ACKs was shown to be essential for the nuclear localization of ACKs. Overexpression of ACK42 (a Cdc42 binding domain of ACK) inhibited cell growth and movement, indicating that Cdc42 signals are transduced to the nucleus through ACKs. This is the first report providing evidence of a novel role for ACKs in transducing Cdc42 signals directly to the nucleus.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011505 Protein-Tyrosine Kinases Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors. Tyrosine Protein Kinase,Tyrosine-Specific Protein Kinase,Protein-Tyrosine Kinase,Tyrosine Kinase,Tyrosine Protein Kinases,Tyrosine-Specific Protein Kinases,Tyrosylprotein Kinase,Kinase, Protein-Tyrosine,Kinase, Tyrosine,Kinase, Tyrosine Protein,Kinase, Tyrosine-Specific Protein,Kinase, Tyrosylprotein,Kinases, Protein-Tyrosine,Kinases, Tyrosine Protein,Kinases, Tyrosine-Specific Protein,Protein Kinase, Tyrosine-Specific,Protein Kinases, Tyrosine,Protein Kinases, Tyrosine-Specific,Protein Tyrosine Kinase,Protein Tyrosine Kinases,Tyrosine Specific Protein Kinase,Tyrosine Specific Protein Kinases
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell

Related Publications

Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
June 2022, The Journal of biological chemistry,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
January 2002, Journal of cellular biochemistry,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
October 1997, The Journal of biological chemistry,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
March 1999, The Journal of biological chemistry,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
December 2019, Biochemical Society transactions,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
November 2009, Clinical cancer research : an official journal of the American Association for Cancer Research,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
January 2017, Science signaling,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
April 1981, Experimental cell research,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
February 2002, Cell structure and function,
Ijaz Ahmed, and Yolanda Calle, and Mohammed A Sayed, and Jabeen M Kamal, and Padmanabhan Rengaswamy, and Ed Manser, and Sally Meiners, and Alam Nur-E-Kamal
July 2013, Cold Spring Harbor perspectives in biology,
Copied contents to your clipboard!