Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores. 2004

Analía I Sannazzaro, and Cora L Alvarez, and Ana B Menéndez, and Fernando L Pieckenstain, and Edgardo O Albertó, and Oscar A Ruiz
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Consejo Nacional de Investigaciones Científicas y Técnicas, Chascomús, Buenos Aires, Argentina.

The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea. Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors alpha-difluoromethylarginine and alpha-difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine, slightly decreased polyamine levels. However, only the latter interfered with spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed.

UI MeSH Term Description Entries
D009955 Ornithine Decarboxylase A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine. Ornithine Carboxy-lyase,Carboxy-lyase, Ornithine,Decarboxylase, Ornithine,Ornithine Carboxy lyase
D011073 Polyamines Amine compounds that consist of carbon chains or rings containing two or more primary amino groups. Polyamine
D011436 Spermidine Synthase An enzyme that catalyzes the transfer of the propylamine moiety from 5'-deoxy-5'-S-(3-methylthiopropylamine)sulfonium adenosine to putrescine in the biosynthesis of spermidine. The enzyme has a molecular weight of approximately 73,000 kDa and is composed of two subunits of equal size. Aminopropyltransferase,Propylamine Transferase,Spermidine Synthetase,Putrescine Aminopropyltransferase,Aminopropyltransferase, Putrescine,Synthase, Spermidine,Synthetase, Spermidine,Transferase, Propylamine
D002262 Carboxy-Lyases Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1. Carboxy-Lyase,Decarboxylase,Decarboxylases,Carboxy Lyase,Carboxy Lyases
D003514 Cyclohexylamines A family of alicyclic hydrocarbons containing an amine group with the general formula R-C6H10NH2. Cyclohexylamine
D004791 Enzyme Inhibitors Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D005658 Fungi A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies. Fungi, Filamentous,Molds,Filamentous Fungi,Filamentous Fungus,Fungus,Fungus, Filamentous,Mold
D013172 Spores, Fungal Reproductive bodies produced by fungi. Conidia,Fungal Spores,Conidium,Fungal Spore,Spore, Fungal
D045868 Sorghum A plant genus of the family POACEAE. The grain is used for FOOD and for ANIMAL FEED. This should not be confused with KAFFIR LIME or with KEFIR milk product. Johnsongrass,Kaffir Corn,Kafir,Sorghum bicolor,Sorghum halepense,Sudangrass,Corn, Kaffir,Corns, Kaffir,Johnsongrasses,Kaffir Corns,Kafirs,Sorghum bicolors,Sorghum halepenses,Sorghums,Sudangrasses,bicolors, Sorghum,halepenses, Sorghum
D029921 Trifolium A plant genus of the family FABACEAE. Clover, Red,Red Clover,Trifolium pratense,Trifolium repens,Trifolium pratenses,Trifolium repen,Trifoliums,pratenses, Trifolium,repen, Trifolium

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