[Determination of six p-hydroxybenzoic acid esters in laver (nori) by HPLC]. 2003

Yoko Kawasaki, and Chikako Yomota, and Kenichi Tanamoto
yoko@nihs.go.jp

A rapid and simple method was developed for simultaneous determination of methyl, ethyl, isopropyl, npropyl, isobuthyl and n-buthyl p-hydroxybenzoic acid esters (PHBA-Es) in laver by HPLC. Six PHBA-Es were extracted from laver with n-hexane-ethyl acetate (1:1) by shaking. The extract was evaporated. The residue was dissolved in methyl alcohol and determined by HPLC. Recoveries of six PHBA-Es spiked in laver were 93.6-101.2% at the level of 2 micrograms/g.

UI MeSH Term Description Entries
D010226 Parabens Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872) 4-Hydroxybenzoic Acids,Paraben,para-Hydroxybenzoic Acids,4 Hydroxybenzoic Acids,para Hydroxybenzoic Acids
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005504 Food Analysis Measurement and evaluation of the components of substances to be taken as FOOD. Analysis, Food,Analyses, Food,Food Analyses
D012624 Seaweed Multicellular marine macroalgae including some members of red (RHODOPHYTA), green (CHLOROPHYTA), and brown (PHAEOPHYCEAE) algae. They are widely distributed in the ocean, occurring from the tide level to considerable depths, free-floating (planktonic) or anchored to the substratum (benthic). They lack a specialized vascular system but take up fluids, nutrients, and gases directly from the water. They contain CHLOROPHYLL and are photosynthetic, but some also contain other light-absorbing pigments. Many are of economic importance as FOOD, fertilizer, AGAR, potash, or source of IODINE. Macroalgae

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