BIOMAT Database Logo BIOMAT Database Logo

Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1-derived O-glycans. 2004

Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, 825 N.E. 13th St., Oklahoma City, OK 73104, USA. lijun-xia@omrf.ouhsc.edu

The core 1 beta1-3-galactosyltransferase (T-synthase) transfers Gal from UDP-Gal to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 1 O-glycan Galbeta1-3GalNAcalpha1-Ser/Thr (T antigen). The T antigen is a precursor for extended and branched O-glycans of largely unknown function. We found that wild-type mice expressed the NeuAcalpha2-3Galbeta1-3GalNAcalpha1-Ser/Thr primarily in endothelial, hematopoietic, and epithelial cells during development. Gene-targeted mice lacking T-synthase instead expressed the nonsialylated Tn antigen in these cells and developed brain hemorrhage that was uniformly fatal by embryonic day 14. T-synthase-deficient brains formed a chaotic microvascular network with distorted capillary lumens and defective association of endothelial cells with pericytes and extracellular matrix. These data reveal an unexpected requirement for core 1-derived O-glycans during angiogenesis.

UI MeSH Term Description Entries
D008833 Microcirculation The circulation of the BLOOD through the MICROVASCULAR NETWORK. Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D001777 Blood Coagulation The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot. Blood Clotting,Coagulation, Blood,Blood Clottings,Clotting, Blood
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005260 Female Females
D005700 Galactosyltransferases Enzymes that catalyze the transfer of galactose from a nucleoside diphosphate galactose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-. Galactosyltransferase
D005865 Gestational Age The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination. Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D006031 Glycosylation The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION). Protein Glycosylation,Glycosylation, Protein

Related Publications

Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Premature ovarian failure in mice with oocytes lacking core 1-derived O-glycans and complex N-glycans.
March 2011, Endocrinology,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Loss of intestinal core 1-derived O-glycans causes spontaneous colitis in mice.
April 2011, The Journal of clinical investigation,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Loss of Core 1-derived O-Glycans Decreases Breast Cancer Development in Mice.
August 2015, The Journal of biological chemistry,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Defective angiogenesis in mice lacking endoglin.
May 1999, Science (New York, N.Y.),
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Fatal hemorrhage in mice lacking gamma-glutamyl carboxylase.
June 2007, Blood,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Core 1-derived O-glycans are essential E-selectin ligands on neutrophils.
May 2010, Proceedings of the National Academy of Sciences of the United States of America,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1.
November 2007, Biochemical and biophysical research communications,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Mice lacking core 1-derived O-glycan in podocytes develop transient proteinuria, resulting in focal segmental glomerulosclerosis.
March 2020, Biochemical and biophysical research communications,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Core 1- and 3-derived O-glycans collectively maintain the colonic mucus barrier and protect against spontaneous colitis in mice.
January 2017, Mucosal immunology,
Lijun Xia, and Tongzhong Ju, and Andrew Westmuckett, and Guangyu An, and Lacramioara Ivanciu, and J Michael McDaniel, and Florea Lupu, and Richard D Cummings, and Rodger P McEver
Targeted disruption of the gene encoding core 1 beta1-3-galactosyltransferase (T-synthase) causes embryonic lethality and defective angiogenesis in mice.
January 2006, Methods in enzymology,
Copied contents to your clipboard!