Crystallization and preliminary X-ray crystallographic studies on recombinant rat choline acetyltransferase. 2004

Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
Department of Medicinal Chemistry, McKnight Brain Institute and University of Florida, Gainesville, Florida 32610, USA.

Choline acetyltransferase (ChAT) catalyzes the biosynthesis of the neurotransmitter acetylcholine from acetyl-CoA and choline in cholinergic neurons. Rat ChAT (rChAT) was overexpressed in Escherichia coli, purified by affinity chromatography and crystallized. Diffraction data were collected from a single crystal under cryoconditions at the F1 beamline at the Cornell High Energy Synchrotron Source, with a maximal useful diffraction pattern to 1.55 A resolution. The crystals were shown to belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 138.97, b = 77.67, c = 59.67 A and a scaling R(sym) of 0.054 for 72 446 unique reflections. Packing considerations indicate there to be one molecule per asymmetric unit. It is expected that in the near future the structure of rChAT will be obtained using molecular-replacement methods. Elucidation of the structure of rChAT will aid in the development of therapeutic agents for Alzheimer's disease.

UI MeSH Term Description Entries
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002795 Choline O-Acetyltransferase An enzyme that catalyzes the formation of acetylcholine from acetyl-CoA and choline. EC 2.3.1.6. Choline Acetylase,Choline Acetyltransferase,Acetylase, Choline,Acetyltransferase, Choline,Choline O Acetyltransferase,O-Acetyltransferase, Choline
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017356 Synchrotrons Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant. Synchrotron
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies

Related Publications

Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
June 1996, Proteins,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
March 2013, Acta crystallographica. Section F, Structural biology and crystallization communications,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
June 2003, Acta crystallographica. Section D, Biological crystallography,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
March 2001, Acta crystallographica. Section D, Biological crystallography,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
August 2011, Acta crystallographica. Section F, Structural biology and crystallization communications,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
June 1995, Protein science : a publication of the Protein Society,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
August 2000, Acta crystallographica. Section D, Biological crystallography,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
June 2002, Protein and peptide letters,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
February 2005, Acta crystallographica. Section F, Structural biology and crystallization communications,
Wei Lian, and Yunrong Gu, and Brenda Pedersen, and Thomas Kukar, and Lakshmanan Govindasamy, and Mavis Agbandje-McKenna, and Shouguang Jin, and Robert McKenna, and Donghai Wu
August 2002, Acta crystallographica. Section D, Biological crystallography,
Copied contents to your clipboard!