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A collaborative study between two laboratories has been carried out in order to investigate the reproducibility of the cytokinesis-block micronucleus assay in human lymphocytes. A modified protocol to obtain good quality slides from whole blood cultures has been developed. The spontaneous frequencies of micronuclei in binucleated lymphocytes from five male subjects were evaluated in each laboratory at two cytochalasin-B concentrations (3 and 6 micrograms/ml). Our data show that 6 micrograms/ml cytochalasin-B was more effective than 3 micrograms/ml to arrest cytokinesis in all the donors analysed. The mean frequency of micronuclei in binucleated cells was significantly lower at 6 micrograms/ml compared to 3 micrograms/ml cytochalasin-B in both laboratories. The results obtained indicate a very high concordance between both sets of data supporting the validity of the proposed protocol.
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E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
August 2006,
Mutation research,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
February 1993,
Mutation research,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
May 1990,
Ceskoslovenska farmacie,
J Surrallés, and
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C Tanzarella
February 1988,
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A Antoccia, and
C Tanzarella
November 1986,
Mutation research,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
March 1986,
Zhonghua zhong liu za zhi [Chinese journal of oncology],
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
September 2006,
Mutation research,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
January 1989,
Radiobiologiia,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
July 2005,
Environmental and molecular mutagenesis,
J Surrallés, and
E Carbonell, and
R Marcos, and
F Degrassi, and
A Antoccia, and
C Tanzarella
December 2005,
Environmental and molecular mutagenesis,
