Biomaterial Database

Metallothionein-like protein in the least killifish Heterandria formosa and its role in cadmium resistance. 2004

Lingtian Xie, and Paul L Klerks

Department of Biology, University of Louisiana at Lafayette, P. O. Box 42451, Lafayette, Louisiana 70504, USA.

Metallothioneins (MTs) are a class of low molecular weight, cysteine-rich, metal-binding proteins ubiquitous in animals. They function in metal regulation and detoxification. An MT-like protein was separated by gel-permeation high-performance liquid chromatography (HPLC) in the least killifish Heterandria formosa exposed to 6 mg/L of Cd for 26 h. Western blot analysis showed that this protein cross-reacted with a cod-MT antibody. We quantified MT-like protein levels by the Cd-hemoglobin saturation assay in fish from two Cd-resistant and two control laboratory populations. Fish from the resistant populations had higher MT-like protein levels than those from the control populations; this difference was found for basal levels and for levels after 26 h of exposure to Cd. Our results suggest that the MT-like protein detoxified Cd in the least killifish and accounted for at least part of the increased resistance in the Cd-resistant laboratory populations.

UI	MeSH Term	Description	Entries
D008668	Metallothionein	A low-molecular-weight (approx. 10 kD) protein occurring in the cytoplasm of kidney cortex and liver. It is rich in cysteinyl residues and contains no aromatic amino acids. Metallothionein shows high affinity for bivalent heavy metals.	Isometallothionein,Metallothionein A,Metallothionein B,Metallothionein I,Metallothionein II,Metallothionein IIA
D002104	Cadmium	An element with atomic symbol Cd, atomic number 48, and atomic weight 112.41. It is a metal and ingestion will lead to CADMIUM POISONING.
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004351	Drug Resistance	Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.	Resistance, Drug
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014873	Water Pollutants	Substances or organisms which pollute the water or bodies of water. Use for water pollutants in general or those for which there is no specific heading.	Water Pollutant,Pollutant, Water,Pollutants, Water
D015153	Blotting, Western	Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.	Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings