-transparent.png){kind=logo}
It has been found that the double bond of free, unreacted acrylamide in a gel can react with a free -SH group of proteins, forming a cysteinyl-S-propionamide adduct. When beta-lactoglobulin was incubated at concentration levels lower than those of free acrylamide, left after polymerizing a 5% T, 4% C gel (barely 12 mM), under anaerobic conditions in 0.1 M borate, pH 9.5, for 1 h and then the tryptic digests analyzed by high performance liquid chromatography (HPLC), two new peptides were detected. The two new peaks were recovered and sequenced by the Edman degradation procedure. They correspond to the sequence from Leu-149 to Ile-162. Residue No. 160 was found to be a cysteinyl-S-propionamide reaction product. Interestingly, only this residue, out of a total of 5 Cys residues, had reacted. No other amino acids (including -NH2 terminus and free -NH2 in Lys) reacted with free acrylamide. The addition of free acrylamide to the -SH group could be completely inhibited if: (i) the gel was extensively washed prior to sample application, or (ii) the gel was incubated for 1 h in 100 mM free Cys.
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
July 1983,
Bioorganicheskaia khimiia,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
February 1987,
FEBS letters,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
August 1980,
Biochimica et biophysica acta,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
June 1974,
Thrombosis et diathesis haemorrhagica,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
February 1967,
Ceskoslovenska farmacie,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
January 1963,
Biokhimiia (Moscow, Russia),
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
January 1974,
Sudebno-meditsinskaia ekspertiza,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
June 1984,
Clinical biochemistry,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
March 1936,
The Journal of general physiology,
M Chiari, and
P G Righetti, and
A Negri, and
F Ceciliani, and
S Ronchi
December 1969,
Biochemistry,
