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Quantitative analysis of MEL-14 expression on various lymphocyte subpopulations. 1992

J Ohgama, and K Onoé
Section of Pathology, Hokkaido University, Sapporo, Japan.

Amounts of gp90MEL-14 molecules in various lymphocyte subpopulations were analyzed. The expression of gp90MEL-14 was rapidly up-regulated in the course of usual procedures for preparing single cell suspensions. On the other hand, the gp90MEL-14 expression was down-modulated after short term culture in the presence of PMA. Addition of NaN3 in the medium for preparing cell suspension was shown to stabilize the expression profile of the gp90MEL-14. By this simple improvement, we could successfully evaluate the precise amounts of gp90MEL-14 expressed on various lymphoid cells which appeared to reflect the expression pattern of gp90MEL-14 in vivo. The proportion of MEL-14+ cells and amounts of gp90MEL-14 on the cells were clearly associated with the anatomical and functional distance from endothelial cells of HEV in peripheral lymph nodes. The present findings, thus, support the role of gp90MEL-14 molecules as homing receptors for HEV that was originally proposed by Weissman and his colleagues.

UI MeSH Term Description Entries
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D010581 Peyer's Patches Lymphoid tissue on the mucosa of the small intestine. Patches, Peyer's,Peyer Patches,Peyers Patches
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D001386 Azides Organic or inorganic compounds that contain the -N3 group. Azide
D013154 Spleen An encapsulated lymphatic organ through which venous blood filters.

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