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A method was developed for the rapid determination of testosterone in urine. The procedure consists of solid-phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) clean-up before gas chromatographic determination. Recovery was evaluated by adding [3H]testosterone (10(4) cpm) to urine samples; the mean recovery of radioactivity after SPE and HPLC was 82%. Precision was estimated by repeated measurement of testosterone in four different urine samples; the coefficient of variation was 7.9% (95% confidence limits 6.1-11.4%). Accuracy was evaluated by standard addition and dilution assays; a linear relationship was found between the expected and observed values (r2 = 0.982). The method is rapid, effective and suitable for routine analysis.
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
January 1999,
The International journal of biological markers,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
January 2002,
The International journal of biological markers,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
August 2001,
Biological & pharmaceutical bulletin,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
February 1997,
Journal of chromatography. B, Biomedical sciences and applications,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
June 2008,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
March 1988,
Journal of chromatography,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
May 1990,
Journal of chromatography,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
January 1991,
Journal of pharmaceutical and biomedical analysis,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
May 2009,
Se pu = Chinese journal of chromatography,
E Venturelli, and
A Manzari, and
A Cavalleri, and
M Benzo, and
G Secreto, and
E Marubini
September 2011,
Journal of biomedical research,
