OBJECTIVE The purpose of this vivo study was to compare fluoride concentration in saliva of patients treated with fluoride varnish and fluoride solution. METHODS 20 5-year-old children were selected and divided into fluoride varnish and fluoride solution groups. The samples of unstimulated whole saliva were collected at base-line before and after 0.25h,0.5h,1h,2h,6h,12h,24h treatment with fluoride varnish and fluoride solution. Saliva fluoride concentrations were measured using an ion-specific electrode. RESULTS After 0.25h,0.5h,1h,2h,6h treatment with fluoride varnish, the values of salivary fluoride concentration were higher than baseline,the values of fluoride varnish group were higher than those in fluoride solution group after 0.5h to 6h. CONCLUSIONS Compared with fluoride solution, fluoride varnish maintained a longer time of high salivary fluoride concentration.