Biomaterial Database

Biochemical and physicochemical changes in collagen isolated from the gall bladder of gall stone patients. 2002

A Geetha

Postgraduate Department of Biochemistry, Bharathi Women's College (Autonomous), North Chennai, Tamil Nadu, India.

Abnormal gall bladder motor function with delayed emptying and stasis are the contributory factors of gall stone formation. Since collagen is the major contractile protein, this study was designed to find out whether the biochemical and physicochemical changes of collagen contribute to the pathogenesis of gall stone formation. Collagen was isolated from the gall bladder of 25 gall stone patients undergoing cholecystectomy and from that of 20 gall stone free subjects. The levels of total, soluble and insoluble collagen were determined. The activity levels of collagenase (3.4.23.3) and protease (3.4.24.11) were assessed. Levels of susceptibility of collagen to denaturing agents 2 M potassium thiocyanate and 8 M urea were estimated. Aldehyde content, shrinkage temperature and tensile strength were also determined in isolated collagen. SDS-PAGE was carried out and alpha, beta fractions were quantified. The total and insoluble collagen contents were significantly high in gall stone patients. The activity levels of collagenase and protease were significantly low. Elevated levels of lipid peroxides, aldehyde content, shrinkage temperature and tensile strength were observed in gall stone patients. There is a significant elevation in the beta fraction and a decrease in alpha/beta ratio. Ultramicroscopic structure of gall bladder revealed derangement of collagen fibres and altered tissue architecture. The results showed that the qualitative and quantitative alterations in collagen also contribute for the defective contractility and stasis of gall bladder in gall stone patients.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D003094	Collagen	A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).	Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D005260	Female		Females
D005704	Gallbladder	A storage reservoir for BILE secretion. Gallbladder allows the delivery of bile acids at a high concentration and in a controlled manner, via the CYSTIC DUCT to the DUODENUM, for degradation of dietary lipid.	Gallbladders
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D013718	Tensile Strength	The maximum stress a material subjected to a stretching load can withstand without tearing. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p2001)	Strength, Tensile,Strengths, Tensile,Tensile Strengths