An automated turbidimetric method for the identification of certain antibiotic groups in incurred kidney samples. 2004

Anna-Liisa Myllyniemi, and Lasse Nuotio, and Erja Lindfors, and Hannu Korkeala
Department of Bacteriology, PO Box 45 (Hämeentie 57), Helsinki FIN-00581, Finland. anna-liisa.myllyniemi@eela.fi

An automated turbidimetric method was developed for group level identification of penicillinase sensitive penicillins, tetracyclines and fluoroquinolones in kidney samples. A sample pretreatment procedure was elaborated for the extraction of incurred residues from kidney tissue in a translucent solution to enable the measurement of changes in optical density. The method was comprised of three pairs, one for each antibiotic group: a sensitive test bacterium strain and a resistant strain for the identification of fluoroquinolones and tetracyclines, and a sensitive strain with and without penicillinase for the identification of penicillinase sensitive penicillins. The algorithm employed compared the areas under the OD vs. time curves; threshold values and experimentally observed intra-test criteria were also included in the algorithm. Antibiotics were reliably identified to group level, and no false identifications were obtained with antibiotics belonging to groups not included in the reference panel. Incurred penicillin G, oxytetracycline and enrofloxacin-ciprofloxacin residues were identified at or below the MRL levels for kidney tissue. The graphically determined shortest possible identification times varied between 2 and 7 h. The method developed could furthermore easily be diversified to include other antibiotic groups by adding new "sensitive-resistant" bacterium and medium combinations.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D009391 Nephelometry and Turbidimetry Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam. Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000465 Algorithms A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task. Algorithm
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial

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