BIOMAT Database Logo BIOMAT Database Logo

Protection against systemic candidiasis in mice immunized with secreted aspartic proteinase 2. 2004

Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Laboratório de Imunologia Mário Arala Chaves, Instituto de Ciências Biomédicas de Abel Salazar, Largo do Prof. Abel Salazar 2, 4099-003 Porto, Portugal. vilanova@icbas.up.pt

Secreted aspartic proteinases (Sap) have been described as virulence factors implicated in the mechanisms of host colonization by the yeast Candida albicans in different types of candidiasis. Intraperitoneal inoculation of C. albicans into BALB/c mice rapidly leads to systemic candidiasis, with significant colonization of the kidneys measurable in the following week. In this study we assessed the potential of vaccination with C. albicans secreted aspartic proteinase 2 (Sap2) in preventing systemic candidiasis in BALB/c mice. Intradermal injection of highly purified native Sap2 protein incorporated in alum adjuvant provided efficient immune protection, as indicated by a 20-fold decrease in the colonization of kidneys. The protective effect of Sap2 immunization with alum adjuvant was also observed in mice infected with a lethal inoculum of C. albicans. Immunization with the native Sap2 alone, as well as with a denatured recombinant form of the protein, also conferred protection, albeit to a lesser level. In all cases, protection correlated with an increase in serum antibodies to Sap2. Moreover, passive transfer of anti-Sap2 immunoglobulin G (IgG) significantly decreased the yeast burden in kidneys of C. albicans-infected mice. This result shows that immune protection against systemic candidiasis in mice immunized with Sap2 is antibody-mediated. Taken together, these analyses demonstrate that Sap2 can be successfully used as a vaccination target in systemic candidiasis and reveals the potential immunomodulatory role of Sap2 on C. albicans infection.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007116 Immunization, Passive Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER). Convalescent Plasma Therapy,Immunoglobulin Therapy,Immunotherapy, Passive,Normal Serum Globulin Therapy,Passive Antibody Transfer,Passive Transfer of Immunity,Serotherapy,Passive Immunotherapy,Therapy, Immunoglobulin,Antibody Transfer, Passive,Passive Immunization,Therapy, Convalescent Plasma,Transfer, Passive Antibody
D007271 Injections, Intradermal The forcing into the skin of liquid medication, nutrient, or other fluid through a hollow needle, piercing the top skin layer. Intradermal Injections,Injection, Intradermal,Intradermal Injection
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D002177 Candidiasis Infection with a fungus of the genus CANDIDA. It is usually a superficial infection of the moist areas of the body and is generally caused by CANDIDA ALBICANS. (Dorland, 27th ed) Candida Infection,Moniliasis,Candida Infections,Candidiases,Infection, Candida,Moniliases
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal

Related Publications

Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Systemic candidiasis in mice immunized with Candida albicans ribosomes.
July 1985, Mycopathologia,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Vaccination with Secreted Aspartyl Proteinase 2 Protein from Candida parapsilosis Can Enhance Survival of Mice during C. tropicalis-Mediated Systemic Candidiasis.
September 2020, Infection and immunity,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Absence of correlation between delayed-type hypersensitivity and protection in experimental systemic candidiasis in immunized mice.
January 1981, Infection and immunity,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Immunoprotection against systemic candidiasis in mice.
May 1995, International immunology,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Secreted aspartic proteinase family of Candida tropicalis.
January 2001, Infection and immunity,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Protein A induced protection against experimental candidiasis in mice.
January 1997, Mycopathologia,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
cDNA cloning of an aspartic proteinase secreted by Candida albicans.
January 1992, Microbiology and immunology,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Antibody responses and protection in mice immunized orally against influenza virus.
December 1996, Vaccine,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Expression of Candida albicans secreted aspartyl proteinase in acute vaginal candidiasis.
June 2007, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,
Manuel Vilanova, and Luzia Teixeira, and Iris Caramalho, and Egídio Torrado, and Andreia Marques, and Pedro Madureira, and Adília Ribeiro, and Paula Ferreira, and Miguel Gama, and Jocelyne Demengeot
Profile of Candida albicans-secreted aspartic proteinase elicited during vaginal infection.
March 2005, Infection and immunity,
Copied contents to your clipboard!