[Isolation of polysaccharides from callus culture of Lemna minor L]. 2004

E A Giunter, and O V Popeĭko, and Iu S Ovodov
Institute of Physiology, Komi Research Center, Ural Division of the Russian Academy of Sciences, Syktyvkar, 167982 Russia. elena@physiol.komisc.ru

Two fractions that included acid arabinogalactan and pectin were extracted from the callus culture of duckweed plants (Lemna minor L.) with water and ammonium oxalate. Residues of galactose and arabinose in the 2.0-2.5:1 ratio were the major constituents of acid arabinogalactan. The pectin fraction contained primarily residues of glucuronic acids, galactose, and arabinose. The percentage of arabinogalactan and pectin was similar. The yield of polysaccharide fractions did not depend on the method for their isolation. Extraction with water, treatment of the biomass with an aqueous solution of formalin and diluted hydrochloric acid, and extraction with an aqueous solution of ammonium oxalate allowed us to obtain the highest-purity pectin polysaccharide.

UI MeSH Term Description Entries
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D002849 Chromatography, Gas Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D029064 Araceae A plant family of the order ALISMATALES. Many members contain OXALIC ACID and calcium oxalate (OXALATES). Cabbage, Skunk,Dracunculus Plant,Lettuce, Water,Lysichiton,Monstera,Orontium,Pistia,Skunk Cabbage,Symplocarpus,Aroideae,Aroids,Pistia stratiotes,Rhaphidophora,Aroid,Cabbages, Skunk,Dracunculus Plants,Lettuces, Water,Lysichitons,Monsteras,Orontiums,Pistia stratiote,Pistias,Plant, Dracunculus,Plants, Dracunculus,Rhaphidophoras,Skunk Cabbages,Water Lettuce,Water Lettuces,stratiote, Pistia

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