-transparent.png){kind=logo}
Highly purified peroxisomal membranes stripped from their peripheral membrane proteins and only minimally contaminated with other membranes, contained three GTP-binding proteins of 29, 27 and 25 kDa, respectively. Bound radioactive GTP was displaced by unlabelled GTP, GTP analogs and GDP but not by GMP or other nucleotides. GTP binding was markedly decreased by trypsin treatment of intact purified peroxisomes; it increased 2-3-fold after pretreatment of the animals with a peroxisome proliferator. We conclude that the peroxisomal membrane contains small GTP-binding proteins that are exposed to the cytosol and that are firmly anchored in the membrane. We speculate that these proteins are involved in peroxisome multiplication by fission or budding during peroxisome biogenesis and proliferation.
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
September 1994,
Plant physiology,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
January 1992,
International review of cytology,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
October 1989,
Trends in biochemical sciences,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
January 2001,
Physiological reviews,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
January 1993,
The International journal of biochemistry,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
February 1997,
Experimental eye research,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
May 1995,
Plant physiology,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
February 1990,
Proceedings of the National Academy of Sciences of the United States of America,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
November 1991,
FEBS letters,
K Verheyden, and
M Fransen, and
P P Van Veldhoven, and
G P Mannaerts
January 1996,
Biology of the cell,
