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Elevated cell calcium has been implicated in functional changes with human erythrocyte aging. However, until recently it has been difficult to measure free ionic intracellular calcium in red cells. We have made use of a fluorinated calcium chelator probe (5,5'-difluoroBAPTA) and fluorine nuclear magnetic resonance (19F-NMR) techniques to measure changes of intracellular Ca2+ concentrations ([Ca2+]i) with cell aging. We have demonstrated in these studies that human erythrocyte [Ca2+]i is significantly elevated as a function of in-vivo aging. Young cells, the least dense fraction of density-separated erythrocytes, contained an average of 62 (+/- 4) nM Ca2+ (+/- S.E.), whereas the oldest, most dense cell fraction contained 221 nM Ca2+ (+/- 25). Mechanisms by which intracellular [Ca2+] increases with in-vivo aging are currently under investigation.
N R Aiken, and
J D Satterlee, and
W R Galey
July 1981,
Analytical biochemistry,
N R Aiken, and
J D Satterlee, and
W R Galey
May 1996,
Magnetic resonance in medicine,
N R Aiken, and
J D Satterlee, and
W R Galey
June 1991,
Magnetic resonance in medicine,
N R Aiken, and
J D Satterlee, and
W R Galey
January 2019,
Journal of visualized experiments : JoVE,
N R Aiken, and
J D Satterlee, and
W R Galey
March 1990,
Environmental health perspectives,
N R Aiken, and
J D Satterlee, and
W R Galey
September 1988,
Circulation research,
N R Aiken, and
J D Satterlee, and
W R Galey
September 1993,
Archives of biochemistry and biophysics,
N R Aiken, and
J D Satterlee, and
W R Galey
December 1990,
Magnetic resonance in medicine,
N R Aiken, and
J D Satterlee, and
W R Galey
January 1996,
Advances in experimental medicine and biology,
N R Aiken, and
J D Satterlee, and
W R Galey
August 1987,
Proceedings of the National Academy of Sciences of the United States of America,
