-transparent.png){kind=logo}
A method for the detection by electron microscopy of chromosome banding after in situ hybridization of small, nonradioactive DNA sequences is described. Typical high-resolution G-banding is produced by adding 5-bromodeoxyuridine (BrdU) during the last part of the S-phase and by applying a monoclonal antibody against the BrdU-substituted chromosome segments, followed by the addition of protein G, but no further treatment. A protocol for in situ hybridization of small, single-copy biotinylated DNA sequences and their detection by immunogold tagging on banded chromosomes is also described. This combined approach permits high-resolution mapping of small DNA sequences and should be useful in discriminating between neighboring DNA fragments.
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1983,
Chromosoma,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
May 1988,
Nucleic acids research,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1981,
Chromosoma,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
March 1989,
Human genetics,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1990,
Anticancer research,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
July 1981,
Experimental cell research,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
December 2001,
Archives of histology and cytology,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1994,
Methods in molecular biology (Clifton, N.J.),
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1987,
Genetika,
R Fetni, and
N Lemieux, and
B Malfoy, and
B Dutrillaux, and
P E Messier, and
C L Richer
January 1982,
Human genetics,
