Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells. 2004

Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
Department of Gastroenterology, Shanghai Second Medical University, Xinhua Hospital, 1665 KongJiang Rd., Shanghai 200092, China. fangchunhua@online.sh.cn

OBJECTIVE To assess the effects of somatostatin on proliferation and apoptosis of activated rat hepatic stellate cells (HSCs). METHODS HSCs isolated from the livers of adult Sprague-Dawley rats (weighing 400-500 g) by in situ perfusion and purified by single-step density gradient centrifugation with Nycodenz, became activated after 10 days' cultivation. Then the apoptotic rate of HSCs treated with different doses of somatostatin for 72 h, was assayed by acridine orange/ethidium bromide fluorescent staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, transmission electron microscopy and flow cytometry, while the proliferation of HSCs was measured by MTT assay. Furthermore, the mechanisms of somatostatin were investigated by cytodynamic analysis. RESULTS Somatostatin at the concentration of 10(-6)-10(-9) mol/L could decrease the proliferative rate, and promote the apoptosis of activated rat HSCs in a dose-dependent way. Its action was most significant when the concentration reached 10(-6) mol/L or 10(-7) mol/L (P<0.05-0.01). An obvious cell-cycle arrest (G(0)/G(1) arrest) was the important way for somatostatin to exert its action. CONCLUSIONS Antiproliferative and proapoptotic effects of low-dose somatostatin on activated rat HSCs can be obtained. These findings reveal its potential antifibrotic action.

UI MeSH Term Description Entries
D008297 Male Males
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013004 Somatostatin A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal. Cyclic Somatostatin,Somatostatin-14,Somatotropin Release-Inhibiting Hormone,SRIH-14,Somatofalk,Somatostatin, Cyclic,Somatotropin Release-Inhibiting Factor,Stilamin,Somatostatin 14,Somatotropin Release Inhibiting Factor,Somatotropin Release Inhibiting Hormone
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D022781 Hepatocytes The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules. Hepatic Cells,Cell, Hepatic,Cells, Hepatic,Hepatic Cell,Hepatocyte

Related Publications

Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
January 2011, Journal of nutritional science and vitaminology,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
April 2011, Food & function,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
January 2014, Cell biochemistry and function,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
June 2007, Planta medica,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
June 2014, International journal of gynecological cancer : official journal of the International Gynecological Cancer Society,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
July 1999, European journal of pharmacology,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
October 2005, Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
October 2010, Fertility and sterility,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
January 2013, PloS one,
Qin Pan, and Ding-Guo Li, and Han-Ming Lu, and Liang-Yong Lu, and Yu-Qin Wang, and Qin-Fang Xu
July 2006, The Journal of pharmacy and pharmacology,
Copied contents to your clipboard!