BIOMAT Database Logo BIOMAT Database Logo

Variants of U1 small nuclear RNA assemble into spliceosomal complexes. 2004

S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Department of Biological Sciences, Florida International University, Miami, FL 33199, USA.

In this study, the existence of additional U1 snRNA variants in the posterior silk gland of the Bombyx mori Nistari strain from India was investigated. Three new U1 variants were detected. One of the new isoforms (U1 SG1) was found to be preferentially assembled into high molecular weight spliceosomal complexes in comparison with the total cellular lysate RNA control. Structural and nucleotide differences were examined in these new isoforms and compared with the previously reported U1 variants. Free energy (Delta G) values for the entire U1 snRNA secondary structures as well as the individual stem/loops (I, II, III and IV) domains of the isoforms were estimated to determine their structural stability.

UI MeSH Term Description Entries
D007194 India A country in southern Asia, bordering the Arabian Sea and the Bay of Bengal, between Burma and Pakistan. The capitol is New Delhi. Republic of India
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012342 RNA, Small Nuclear Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors. Low Molecular Weight Nuclear RNA,Small Nuclear RNA,snRNA,Chromatin-Associated RNA,Small Molecular Weight RNA,Chromatin Associated RNA,RNA, Chromatin-Associated
D012831 Bombyx A genus of silkworm MOTHS in the family Bombycidae of the order LEPIDOPTERA. The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of Asia, it is sometimes reared in this country. It has long been raised for its SILK and after centuries of domestication it probably does not exist in nature. It is used extensively in experimental GENETICS. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p519) Bombyx mori,Silkmoths,Silkworms,Silkmoth,Silkworm,Bombyx morus,Bombyxs,mori, Bombyx
D017381 Spliceosomes Organelles in which the splicing and excision reactions that remove introns from precursor messenger RNA molecules occur. One component of a spliceosome is five small nuclear RNA molecules (U1, U2, U4, U5, U6) that, working in conjunction with proteins, help to fold pieces of RNA into the right shapes and later splice them into the message. Spliceosome
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse

Related Publications

S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
U1 small nuclear RNA and spliceosomal introns in Euglena gracilis.
February 1999, Proceedings of the National Academy of Sciences of the United States of America,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle.
January 2001, Nature,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
U1 small nuclear RNA variants differentially form ribonucleoprotein particles in vitro.
April 2014, Gene,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Spliceosomal introns in conserved sequences of U1 and U5 small nuclear RNA genes in yeast Rhodotorula hasegawae.
September 1996, Journal of biochemistry,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Transcription boundaries of U1 small nuclear RNA.
September 1985, Molecular and cellular biology,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Nuclear ribonucleoprotein complexes containing U1 and U2 RNA.
October 1975, Biochemistry,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation.
January 2011, Wiley interdisciplinary reviews. RNA,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Gene therapy with modified U1 small nuclear RNA.
May 2017, Expert review of endocrinology & metabolism,
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
Spliceosomal small nuclear RNA genes in 11 insect genomes.
January 2007, RNA (New York, N.Y.),
S Pereira-Simon, and J M Sierra-Montes, and K Ayesh, and L Martinez, and A Socorro, and R J Herrera
ISG20 and nuclear exosome promote destabilization of nascent transcripts for spliceosomal U snRNAs and U1 variants.
January 2021, Genes to cells : devoted to molecular & cellular mechanisms,
Copied contents to your clipboard!