A new Olympus assay for the determination of ceruloplasmin. 2004

Tracey C Larkin, and Teresina A Fingleton, and Matthew Mccusker, and Martin Cassidy, and Gerhard Gunzer, and Cyrus A Lepp, and Erby Gamboa
Olympus Diagnostica GmbH (Ireland), Lismeehan, O'Callaghans Mills, Co. Clare, Ireland. tracey.larkin@olympus.ie

Ceruloplasmin's main clinical importance is in the diagnosis of Wilson's disease, where plasma ceruloplasmin concentration is reduced. Increased levels are particularly notable in diseases of the reticuloendothelial system such as Hodgkin's disease as well as during pregnancy or use of contraceptive pills. This paper describes a new Olympus assay OSR6164 for the determination of ceruloplasmin on the Olympus AU640E, AU400E and AU2700 analytical systems. The turbidimetric assay has a linear measurable range of 60-2000 mg/l. Prozone hook effect greater than 9000 mg/l. Imprecision CV values < 5% for within-run and < 10% for between-day. Lipemic, hemolytic and icteric interference of < or = 5%. Traceability of recovery to CRM470 standardisation. Method comparison to Behring Nephelometry yielded a correlation (r) of 0.99 and a slope of 1.02. On-board and calibration stability of 60 days and 14 days, respectively. Serumor plasma (EDTA and lithium heparin anticoagulants) samples may be used.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D009391 Nephelometry and Turbidimetry Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam. Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D002570 Ceruloplasmin A multi-copper blood FERROXIDASE involved in iron and copper homeostasis and inflammation. Caeruloplasmin,Ferroxidase,Ceruloplasmin Ferroxidase,Ceruloplasmin Oxidase,Ferroxidase I,alpha(2)-Ceruloplasmin,Ferroxidase, Ceruloplasmin,Oxidase, Ceruloplasmin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000925 Anticoagulants Agents that prevent BLOOD CLOTTING. Anticoagulant Agent,Anticoagulant Drug,Anticoagulant,Anticoagulant Agents,Anticoagulant Drugs,Anticoagulation Agents,Indirect Thrombin Inhibitors,Agent, Anticoagulant,Agents, Anticoagulant,Agents, Anticoagulation,Drug, Anticoagulant,Drugs, Anticoagulant,Inhibitors, Indirect Thrombin,Thrombin Inhibitors, Indirect
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face

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