An effective morphometric method for electron microscopic studies on papillary muscles. 1978

G Mall, and H Reinhard, and K Kayser, and J A Rossner

Morphometry was performed on the left ventricular posterior papillary muscles of seven Wistar rats. The volume densities of myocardial cells, interstitial space, myocardial nuclei, sarcoplasm, mitochondria, myofibrils, ground substance and T tubules, and the surface densities of myocardial cells, mitochondrial membranes and T tubules, were calculated. Though only 1 ultrathin section per animal was evaluated the low standard errors of the means indicate that the method described here will be adequate in most experimental studies. Due to the anisotropy of the surfaces within myocardial cells, the papillary muscles were cut at an angle of 32.4 degrees to their longitudinal axis. This angle is derived from an equation published by Whitehouse (1974). The procedure to correct the loss of cristal membrane images from oblique sectioning is discussed.

UI MeSH Term Description Entries
D008297 Male Males
D008433 Mathematics The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Mathematic
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008929 Mitochondria, Heart The mitochondria of the myocardium. Heart Mitochondria,Myocardial Mitochondria,Mitochondrion, Heart,Heart Mitochondrion,Mitochondria, Myocardial
D009210 Myofibrils The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES . Myofilaments,Myofibril,Myofilament
D010210 Papillary Muscles Conical muscular projections from the walls of the cardiac ventricles, attached to the cusps of the atrioventricular valves by the chordae tendineae. Muscle, Papillary,Muscles, Papillary,Papillary Muscle
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001699 Biometry The use of statistical and mathematical methods to analyze biological observations and phenomena. Biometric Analysis,Biometrics,Analyses, Biometric,Analysis, Biometric,Biometric Analyses
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1972, Nihon Seikeigeka Gakkai zasshi,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1968, The Anatomical record,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
August 1975, Cell and tissue research,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
July 1993, Journal of the American Society of Nephrology : JASN,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
June 1989, Journal of the American Academy of Dermatology,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
August 1972, Chest,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1969, Pathologia et microbiologia,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1971, Acta medica Polona,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1970, Shinkei kenkyu no shimpo. Advances in neurological sciences,
G Mall, and H Reinhard, and K Kayser, and J A Rossner
January 1961, Acta neurovegetativa,
Copied contents to your clipboard!