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In order to understand the mechanisms that bring about maintenance and restoration of the integrity of corneal epithelium, we investigated independent and combined effects of transforming growth factor-beta (TGF-beta) and epidermal growth factor (EGF) on rabbit corneal epithelial cells in cell and organ culture. Specifically, we determined whether incubation with these factors influenced 1) cellular proliferation, 2) ability of cells to attach to a fibronectin matrix, and 3) the rate of epithelial migration over corneal stroma. Incubation with TGF-beta caused a dose-related decrease in the incorporation of 3H-thymidine by the epithelial cells. EGF increased 3H-thymidine incorporation, but this effect was antagonized by the addition of TGF-beta into the incubation medium. Incubation with EGF increased the numbers of cells that attached to a fibronectin matrix. TGF-beta itself did not affect the number of attached cells but, again, it antagonized the stimulatory effect of EGF. Similarly, when corneal blocks were cultured with EGF, epithelial migration increased in a dose-related manner. TGF-beta itself did not affect epithelial migration at any of the concentrations tested (0.1-10 ng/ml), but it antagonized EGF-stimulated epithelial migration. These findings suggest that the proliferation and the migration of corneal epithelial cells are regulated by different mechanisms, and that TGF-beta serves as a modulator of the effects of EGF.
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
May 2019,
Scientific reports,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
November 1992,
Investigative ophthalmology & visual science,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
January 2020,
International journal of ophthalmology,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
January 1998,
Experimental eye research,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
October 1995,
Experimental cell research,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
November 1989,
Journal of cellular physiology,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
January 1990,
Archives of oral biology,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
May 1985,
The Journal of cell biology,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
February 1992,
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research,
H Mishima, and
M Nakamura, and
J Murakami, and
T Nishida, and
T Otori
January 1988,
Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry,
