Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukaemia. 2004

Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
Department of Pathology, University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China.

EEN (extra eleven nineteen), also known as EA2 (endophilin A2), a fusion partner of the MLL (mixed-lineage leukaemia) gene in human acute leukaemia, is a member of the endophilin A family, involved in the formation of endocytic vesicles. We present evidence to show that EEN/EA2 is localized predominantly in nuclei of various cell lines of haemopoietic, fibroblast and epithelial origin, in contrast with its reported cytoplasmic localization in neurons and osteoclasts, and that EEN/EA2 exhibits nucleocytoplasmic shuttling. During the cell cycle, EEN/EA2 shows dynamic localization: it is perichromosomal in prometaphase, co-localizes with the bipolar spindle in metaphase and anaphase and redistributes to the midzone and midbody in telophase. This pattern of distribution coincides with changes in protein levels of EEN/EA2, with the highest levels being observed in G2/M-phase. Our results suggest that distinct subcellular localization of the endophilin A family members probably underpins their diverse cellular functions and indicates a role for EEN/EA2 in the cell cycle.

UI MeSH Term Description Entries
D007938 Leukemia A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006) Leucocythaemia,Leucocythemia,Leucocythaemias,Leucocythemias,Leukemias
D008938 Mitosis A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D004705 Endocytosis Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis. Endocytoses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
December 2003, Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
March 1991, Biochimica et biophysica acta,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
April 2004, Oncogene,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
May 2000, Experimental cell research,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
January 2007, Blood,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
January 2013, Methods in molecular biology (Clifton, N.J.),
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
November 2017, Molecules and cells,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
October 2006, Leukemia,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
June 2014, British journal of haematology,
Ngai Cheung, and Chi Wai So, and Judy W P Yam, and C K C So, and Randy Y C Poon, and Dong-Yan Jin, and Li Chong Chan
January 2016, American journal of translational research,
Copied contents to your clipboard!