BIOMAT Database Logo BIOMAT Database Logo

Alterations in tumor necrosis factor-induced endothelial cell procoagulant activity by hyperthermia. 2004

Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Surgical Metabolism Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1502, USA.

TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer-bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF-mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF-mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37 degrees C) and hyperthermic (41 degrees C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor-2 (TFPI-2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41 degrees C significantly reduced clotting activity, TF protein and mRNA as well as TFPI-2 mRNA compared to treatment at 37 degrees C. These data show that hyperthermia significantly reduces the procoagulant effects of TNF on endothelial tissue compared to normothermia, which may have important clinical implications for the use of TNF in regional perfusion.

UI MeSH Term Description Entries
D006979 Hyperthermia, Induced Abnormally high temperature intentionally induced in living things regionally or whole body. It is most often induced by radiation (heat waves, infra-red), ultrasound, or drugs. Fever Therapy,Hyperthermia, Local,Hyperthermia, Therapeutic,Thermotherapy,Induced Hyperthermia,Therapeutic Hyperthermia,Therapy, Fever,Local Hyperthermia
D007700 Kinetics The rate dynamics in chemical or physical systems.
D001779 Blood Coagulation Factors Endogenous substances, usually proteins, that are involved in the blood coagulation process. Clotting Factor,Coagulation Factors,Blood Coagulation Factor,Clotting Factors,Coagulation Factor,Coagulation Factor, Blood,Coagulation Factors, Blood,Factor, Coagulation,Factors, Coagulation,Factor, Blood Coagulation,Factor, Clotting,Factors, Blood Coagulation,Factors, Clotting
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Pathophysiologic alterations induced by tumor necrosis factor.
January 1993, International review of experimental pathology,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Effects of hyperthermia and tumour necrosis factor on inflammatory cytokine secretion and procoagulant activity in endothelial cells.
April 2000, Cytokine,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Endothelial cell activation induced by tumor necrosis factor and lymphotoxin.
March 1989, The American journal of pathology,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Platelet-activating factor augments tumor necrosis factor and procoagulant activity.
March 1992, The Journal of surgical research,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Enhancement of tumor necrosis factor-induced endothelial cell injury by cycloheximide.
August 1990, The American journal of physiology,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Procoagulant activity and tumor necrosis factor in rat hepatic allograft rejection.
November 1991, Hepatology (Baltimore, Md.),
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Flavone acetic acid potentiates the induction of endothelial procoagulant activity by tumour necrosis factor.
January 1991, European journal of cancer (Oxford, England : 1990),
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Tumor necrosis factor induced release of endothelial cell lipoprotein lipase.
January 1990, Arteriosclerosis (Dallas, Tex.),
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Mechanism of antitumor activity of tumor necrosis factor alpha with hyperthermia in a tumor necrosis factor alpha-resistant tumor.
December 1990, Journal of the National Cancer Institute,
Nancy M Carroll, and Dina M Elaraj, and Markus Puhlmann, and David M Weinreich, and Ewa M Turner, and Hui Xu, and H Richard Alexander
Tumor necrosis factor and endothelial cell death.
January 1998, Trends in cardiovascular medicine,
Copied contents to your clipboard!