Hydrophilic interaction liquid chromatography-tandem mass spectrometry for the determination of levosulpiride in human plasma. 2004

In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, Republic of Korea.

A rapid, sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric (HILIC-MS/MS) method for the determination of levosulpiride in human plasma was developed. Levosulpiride and internal standard, tiapride were extracted from human plasma with ethyl acetate at pH 11 and analyzed on an Atlantis HILIC silica column with the mobile phase of acetonitrile-ammonium formate (190 mM, pH 3.0) (94:6, v/v). The analytes were detected using an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curve was linear (r = 0.999) over the concentration range of 1.00-200 ng/ml. The lower limit of quantification for levosulpiride was 1.00 ng/ml using 100 microl plasma sample. The coefficient of variation and relative error for intra- and inter-assay at three quality control (QC) levels were 3.8-9.1 and -2.9 to -0.1%, respectively. The recoveries of levosulpiride ranged from 80.5 to 87.4%, with that of tiapride (internal standard) being 84.6%. This method was successfully applied to the pharmacokinetic study of levosulpiride in humans.

UI MeSH Term Description Entries
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013469 Sulpiride A dopamine D2-receptor antagonist. It has been used therapeutically as an antidepressant, antipsychotic, and as a digestive aid. (From Merck Index, 11th ed) Aiglonyl,Arminol,Deponerton,Desisulpid,Digton,Dogmatil,Dolmatil,Eglonyl,Ekilid,Guastil,Lebopride,Meresa,Pontiride,Psicocen,Sulp,Sulperide,Sulpitil,Sulpivert,Sulpor,Synédil,Tepavil,Vertigo-Meresa,neogama,vertigo-neogama,Vertigo Meresa,vertigo neogama
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D021241 Spectrometry, Mass, Electrospray Ionization A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry. ESI Mass Spectrometry,Electrospray Ionization Mass Spectrometry,Mass Spectrometry, ESI,Spectrometry, ESI Mass

Related Publications

In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
May 2006, Journal of pharmaceutical and biomedical analysis,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
November 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
January 2006, Rapid communications in mass spectrometry : RCM,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
August 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
November 2001, Analytical chemistry,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
May 2008, Analytical chemistry,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
May 2009, Rapid communications in mass spectrometry : RCM,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
June 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
April 2017, Biomedical chromatography : BMC,
In Bok Paek, and Ya Moon, and Hye Young Ji, and Hui-Hyun Kim, and Hye Won Lee, and Yong-Bok Lee, and Hye Suk Lee
November 2017, Talanta,
Copied contents to your clipboard!