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[Anti-SARS virus activities of different recombinant human interferons in cell culture system]. 2003
Zhao-jun Duan, and
Li-lan Zhang, and
Zhi-ping Xie, and
Zhi-ai Yu, and
Li-ping Zhang, and
Bin Zhang, and
Yong-qing Liu, and
Jian-wei Wang, and
Wu-ping Li, and
Cheng-hai Zhang, and
Xue-jun Ma, and
Yue-long Shu, and
Shu-min Duan, and
De-xin Li, and
Yun-de Hou
Beijing Jin Di Ke Biotechnology Institute, State Research Center for Biotechnological Engineering, Beijing 100176, China.
BACKGROUND To study the anti-SARS virus activities of different recombinant human interferons on the cell culture system. METHODS Anti-SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda) cell culture. RESULTS The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5+/-129.5) IU/ml, (149.0+/-71.7) IU/ml, (69.5+/-61.5) IU/ml, (87.3+/-47.1) IU/ml, respectively or (0.6+/-0.5) ng/ml, (10.6+/-5.1) ng/ml, (3.5+/-3.1) ng/ml, (0.9+/-0.5) ng/ml, respectively. CONCLUSIONS All the tested recombinant interferons showed anti-SARS virus activities on the Rda cell culture with different sensitivities.
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