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Plasminogen activator and urokinase are often used as biological markers of cell activation. However, the methods currently used are cumbersome, make no discrimination between tissue-type plasminogen activator and urokinase, and do not allow expression of the results of the overall reaction in International Units. The one-step method described in this paper lacks these drawbacks. Moreover, we propose use of H-D-Val-Phe-Lys-4-nitroanilide as substrate which has a lower Km than the standard H-D-Val-Leu-Lys-4-nitroanilide which is commercially available. Low concentrations of sodium dodecyl sulfate in the reaction mixture dramatically and preferentially accelerate the reaction catalyzed by tissue-type plasminogen activators. Identical results are obtained under kinetic or fixed-time assay conditions using either a photometer or 96-well plate reader. The corresponding formulae are provided.
J Schnyder, and
R Marti, and
P H Cooper, and
T G Payne
January 1991,
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March 1990,
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Growth factors (Chur, Switzerland),
J Schnyder, and
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September 1991,
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J Schnyder, and
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July 1994,
Experimental cell research,
J Schnyder, and
R Marti, and
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T G Payne
March 1995,
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J Schnyder, and
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January 1987,
The Journal of investigative dermatology,
J Schnyder, and
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September 1982,
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