Determination of alpha-tocopherol in plasma by high performance liquid chromatography with fluorescence detection and stability of alpha-tocopherol under different conditions. 2004

Andreas Rümelin, and Till Humbert, and Ulrich Fauth
Clinic of Anaesthesiology, Johannes Gutenberg University, Mainz, Germany. ruemelin@mail.uni-mainz.de

In order to study the clinical effects of the antioxidant alpha-tocopherol (tocofersolan, CAS 30999-06-5) a simple, robust, sensitive and reliable high performance liquid chromatography (HPLC) method using fluorescence detection for the daily measurement of alpha-tocopherol concentration in plasma is described. Several methods of HPLC analysis using fluorescence techniques are published, however, investigations of the stability of alpha-tocopherol during sample preparation under different conditions are relevant in the routine measurements and have to date not been reported. Therefore a modified method, both sensitive and robust, qualified for the day-by-day measurement of alpha-tocopherol in plasma was established and the stability of alpha-tocopherol during sample preparation was analysed under different conditions. This method enables the complete separation of alpha-tocopherol from other compounds within 14 min. In addition, investigations on the stability of alpha-tocopherol during sample preparation showed the necessity of rapid sample preparation without delay. Prepared samples can be stored in liquid nitrogen for at least 56 days.

UI MeSH Term Description Entries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D024502 alpha-Tocopherol A natural tocopherol and one of the most potent antioxidant tocopherols. It exhibits antioxidant activity by virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus. It has four methyl groups on the 6-chromanol nucleus. The natural d form of alpha-tocopherol is more active than its synthetic dl-alpha-tocopherol racemic mixture. 3,4-Dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-ol,R,R,R-alpha-Tocopherol,Tocopherol Acetate,Tocopherol Succinate,Tocopheryl Acetate,Vitamin E Succinate,alpha-Tocopherol Acetate,alpha-Tocopherol Hemisuccinate,alpha-Tocopherol Succinate,alpha-Tocopheryl Calcium Succinate,d-alpha Tocopherol,d-alpha-Tocopheryl Acetate,Acetate, Tocopherol,Tocopherol, d-alpha,alpha Tocopherol,alpha Tocopherol Acetate,alpha Tocopherol Hemisuccinate,alpha Tocopherol Succinate,alpha Tocopheryl Calcium Succinate,d alpha Tocopherol,d alpha Tocopheryl Acetate

Related Publications

Andreas Rümelin, and Till Humbert, and Ulrich Fauth
January 1978, Journal of chromatography,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
November 1984, Journal of chromatography,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
April 1983, Analytical biochemistry,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
October 1994, Journal of chromatography. B, Biomedical applications,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
April 1989, Analytical biochemistry,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
January 2000, Se pu = Chinese journal of chromatography,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
April 1996, Journal of chromatography. A,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
September 1982, Journal of chromatography,
Andreas Rümelin, and Till Humbert, and Ulrich Fauth
December 1989, Journal of chromatography,
Copied contents to your clipboard!