Biomaterial Database

Alterations in brain antioxidant status, protein oxidation and lipid peroxidation in response to different stress models. 2004

Emel Sahin, and Saadet Gümüşlü

Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey.

The aim of this study was to investigate the effects of different stress models on copper, zinc-superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (Se-GSH-Px) activities, and reduced glutathione (GSH), protein carbonyl (PC) and lipid peroxidation marker (conjugated diene (CD) and thiobarbituric acid-reactive substances (TBARS)) levels in brain of rats, and to determine the most effective stress model according to each parameter. Rats were divided into four groups as following: control group (C), immobilization stress group (IS), cold stress group (CS) and immobilization-cold stress group (ICS). All stress models increased brain Cu,Zn-SOD and CAT activities, PC, CD and TBARS levels, plasma corticosterone levels and decreased brain GSH concentrations. Se-GSH-Px activity was increased in CS and ICS groups. When all stress models were taken into consideration, the highest increases in Cu,Zn-SOD and Se-GSH-Px activities were found in CS group. The lowest GSH level was seen in IS group. The highest increases in PC and TBARS levels were found in ICS group. The highest increase of CD concentration was seen in IS and ICS groups. Our results suggest that different stress models have different degrees of influences on enzymatic and non-enzymatic antioxidant defense systems, protein oxidation and lipid peroxidation in the brain.

UI	MeSH Term	Description	Entries
D007103	Immobilization	The restriction of the MOVEMENT of whole or part of the body by physical means (RESTRAINT, PHYSICAL) or chemically by ANALGESIA, or the use of TRANQUILIZING AGENTS or NEUROMUSCULAR NONDEPOLARIZING AGENTS. It includes experimental protocols used to evaluate the physiologic effects of immobility.	Hypokinesia, Experimental,Experimental Hypokinesia,Experimental Hypokinesias,Hypokinesias, Experimental
D008297	Male		Males
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D011897	Random Allocation	A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.	Randomization,Allocation, Random
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002374	Catalase	An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.	Catalase A,Catalase T,Manganese Catalase,Mn Catalase
D003080	Cold Temperature	An absence of warmth or heat or a temperature notably below an accustomed norm.	Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D003345	Corticosterone	An adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. (From Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1437)
D005978	Glutathione	A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.	Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine