-transparent.png){kind=logo}
Faecally enriched extracts of Dermatophagoides pteronyssinus were shown to contain a trypsin-like enzyme which was allergenic. Chromatofocusing studies revealed the presence of nine major isoforms in D. pteronyssinus, with pI in the range 4 to greater than 8, but only two (range 4-5) in D. farinae. Trypsin isolated from D. pteronyssinus by benzamidine-Sepharose 6B affinity chromatography and gelfiltration was found to be a 31-kDa protein which was enzymatically similar to both invertebrate and vertebrate trypsins. The N-terminal sequence obtained (IVGGEXALAGEXPYQISL) was identical to that reported for the mite allergen Der p III and showed homology with crayfish trypsin and Der f III from D. farinae. Mite trypsin underwent autolysis and the N-terminal sequences of two fragments were found to be ALAGEXPYQI and NNQVXGI respectively. Both showed homology with crayfish trypsin, and the former sequence was identical to residues 7-18 of the native enzyme and Der p III. All isoforms of mite trypsin were showed to be allergenic by radioallergosorbent assay and further studies indicated that the trypsin degradation products were also allergenic. The enzyme was compared with other mite allergens and the rank order of allergenic potency was shown to be: whole mite extract greater than Der p I greater than trypsin. However, all sera from a panel of mite allergic individuals showed IgE reactivity to trypsin, comparable to that seen using whole mite extract and Der p I. These data indicate that mite trypsin is a major allergen corresponding to the previously described allergen, Der p III.
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
January 1986,
The Journal of allergy and clinical immunology,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
April 1970,
Nederlands tijdschrift voor geneeskunde,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
December 1995,
Immunology letters,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
June 1969,
The Medical journal of Australia,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
May 1992,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
January 1991,
International archives of allergy and applied immunology,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
October 1999,
International archives of allergy and immunology,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
January 1983,
International archives of allergy and applied immunology,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
August 2014,
Insects,
G A Stewart, and
L D Ward, and
R J Simpson, and
P J Thompson
November 1997,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
