Deleteriuos effects of immobilization upon rat skeletal muscle: role of creatine supplementation. 2004

M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
Laboratory of Molecular Biology of Muscle Plasticity, Department of Histology and Embryology, Institute of Biomedical science, USP, Marcelo Saldanha Aoki, Av. Lineu Prestes, 1524., CEP 05508-900 São Paulo, SP, Brazil. msaoki@usp.br

OBJECTIVE The aim of the study was to investigate the impact of creatine feeding (5 g kg(-1) body weight day(-1)) upon the deleterious adaptations in skeletal muscle induced by immobilization. METHODS Male Wistar rats were submitted to hind limb immobilization together with three dietary manipulations: control, supplemented with creatine for 7 days (along with immobilization) and supplemented with creatine for 14 days (7 days before immobilization and together with immobilization). Muscle weight (wet/dry) was determined in the soleus (SOL) and gastrocnemius (GAS). The analysis of lean mass was performed by DEXA and myosin heavy chain (MHC) distribution by SDS-PAGE. RESULTS After 14 days of creatine loading, immobilized SOL and GAS total creatine content were increased by 25% and 18%, respectively. Regardless of dietary manipulation, the immobilization protocol induced a decrease in the weight of SOL and GAS (P < 0.001). However, creatine feeding for 14 days minimized mass loss in the SOL and GAS (P < 0.05). Our findings also indicate that creatine supplementation maximizes the expected slow-to-fast MHC shift driven by immobilization (P < 0.05). CONCLUSIONS Previous creatine supplementation attenuates muscle wasting induced by immobilization. This effect is associated with the increment of intramuscular creatine content.

UI MeSH Term Description Entries
D008297 Male Males
D009133 Muscular Atrophy Derangement in size and number of muscle fibers occurring with aging, reduction in blood supply, or following immobilization, prolonged weightlessness, malnutrition, and particularly in denervation. Atrophy, Muscle,Neurogenic Muscular Atrophy,Neurotrophic Muscular Atrophy,Atrophies, Muscle,Atrophies, Muscular,Atrophies, Neurogenic Muscular,Atrophies, Neurotrophic Muscular,Atrophy, Muscular,Atrophy, Neurogenic Muscular,Atrophy, Neurotrophic Muscular,Muscle Atrophies,Muscle Atrophy,Muscular Atrophies,Muscular Atrophies, Neurogenic,Muscular Atrophies, Neurotrophic,Muscular Atrophy, Neurogenic,Muscular Atrophy, Neurotrophic,Neurogenic Muscular Atrophies,Neurotrophic Muscular Atrophies
D009929 Organ Size The measurement of an organ in volume, mass, or heaviness. Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D003401 Creatine An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018482 Muscle, Skeletal A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles. Anterior Tibial Muscle,Gastrocnemius Muscle,Muscle, Voluntary,Plantaris Muscle,Skeletal Muscle,Soleus Muscle,Muscle, Anterior Tibial,Muscle, Gastrocnemius,Muscle, Plantaris,Muscle, Soleus,Muscles, Skeletal,Muscles, Voluntary,Skeletal Muscles,Tibial Muscle, Anterior,Voluntary Muscle,Voluntary Muscles

Related Publications

M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
April 2005, Journal of applied physiology (Bethesda, Md. : 1985),
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
February 2001, Acta physiologica Scandinavica,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
August 2002, Life sciences,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
August 2007, Life sciences,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
February 1998, Aging (Milan, Italy),
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
September 1995, Cardiovascular research,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
August 2000, The American journal of clinical nutrition,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
November 1996, The American journal of physiology,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
January 2010, Journal of strength and conditioning research,
M S Aoki, and W P Lima, and E H Miyabara, and C H A Gouveia, and A S Moriscot
October 2002, Clinical and experimental pharmacology & physiology,
Copied contents to your clipboard!