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Two types of xylanases (1,4-beta-D-xylan xylanohydrolase, EC 3.2.1.8) were isolated from the culture filtrate of a thermophilic actinomycete, Streptomyces thermoviolaceus OPC-520. The enzymes (STX-I and STX-II) were purified by chromatography with DEAE-Toyopearl 650 M, CM-Toyopearl 650 M, Sephadex G-75, Phenyl-Toyopearl 650 M, and Mono Q HR. The purified enzymes showed single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weights of STX-I and STX-II were 54,000 and 33,000, respectively. The pIs were 4.2 (STX-I) and 8.0 (STX-II). The optimum pH levels for the activity of STX-I and STX-II were pH 7.0. The optimum temperature for the activity of STX-I was 70 degrees C, and that for the activity of STX-II was 60 degrees C. The enzymes were completely inhibited by N-bromosuccinimide. The enzymes degraded xylan, producing xylose and xylobiose as the predominant products, indicating that they were endoxylanases. STX-I showed high sequence homology with the exoglucanase from Cellulomonas fimi (47% homology), and STX-II showed high sequence homology with the xylanase from Bacillus pumilus (46% homology).
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
October 2001,
Protein expression and purification,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
September 1998,
Applied and environmental microbiology,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
June 2002,
Phytochemistry,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
January 1992,
Bioscience, biotechnology, and biochemistry,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
January 1979,
Folia microbiologica,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
October 1997,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
November 1990,
FEBS letters,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
March 1991,
Biological chemistry Hoppe-Seyler,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
February 1980,
Journal of biochemistry,
H Tsujibo, and
K Miyamoto, and
T Kuda, and
K Minami, and
T Sakamoto, and
T Hasegawa, and
Y Inamori
December 1987,
Wei sheng wu xue bao = Acta microbiologica Sinica,
