-transparent.png){kind=logo}
To elucidate the mammalian system for synthesis of cobalamin coenzymes, microsomal NADPH-linked aquacobalamin reductase was purified and characterized. The enzyme was purified about 534-fold over rat liver microsomal fraction in a yield of about 32%. The purified enzyme was homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and had a monomeric molecular weight of 79,000. The purified aquacobalamin reductase showed a high specific activity (about 55 mumol/min per mg protein) of NADPH-cytochrome c (P-450) reductase. About 33% of the NADPH-cytochrome c reductase activity found in the microsomal fraction was recovered in the final purified preparation. The activity ratio of NADPH-cytochrome c reductase/NADPH-linked aquacobalamin reductase was about 5.0 through the purification steps, indicating that the rat liver microsomal NADPH-linked aquacobalamin reductase is the NADPH-cytochrome c reductase.
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
July 1979,
Biochemical pharmacology,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
January 1978,
Archives of biochemistry and biophysics,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
March 1983,
Analytical biochemistry,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
February 1981,
Food and cosmetics toxicology,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
July 1979,
Archives of biochemistry and biophysics,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
March 1985,
Biochemical and biophysical research communications,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
December 1980,
Archives of biochemistry and biophysics,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
October 1976,
Archives internationales de pharmacodynamie et de therapie,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
August 1986,
Endocrinology,
F Watanabe, and
Y Nakano, and
H Saido, and
Y Tamura, and
H Yamanaka
October 1974,
Biochemical and biophysical research communications,
