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A sensitive technique was developed for the quantitative detection of intrathecal production of interleukin-2 (IL-2). Concentrations of IL-2 in paired cerebrospinal fluid (CSF) and serum samples were measured by an enzyme-linked immunosorbent assay using a monoclonal antibody and an affinity purified polyclonal antibody. The assay produced a linear response with respect to IL-2 concentration, and could readily detect levels of IL-2 as low as 1.5 international units/ml. Concentrations of IL-2 in CSF and serum samples were standardised by calculating their ratio to albumin concentration in order to correct for passive transudation of IL-2 across blood-CSF barriers. CSF IL-2/albumin ratios higher than concomitant serum ratios were considered indicative of intrathecal IL-2 production. The technique provides a sensitive, specific, and reproducible method for the determination of in vivo synthesis of IL-2 within the central nervous system.
M K Sharief, and
R Hentges, and
E J Thompson
April 1996,
Analytical and bioanalytical chemistry,
M K Sharief, and
R Hentges, and
E J Thompson
May 2007,
Neuroscience letters,
M K Sharief, and
R Hentges, and
E J Thompson
March 1989,
Tubercle,
M K Sharief, and
R Hentges, and
E J Thompson
May 1992,
Journal of immunological methods,
M K Sharief, and
R Hentges, and
E J Thompson
July 1993,
Steroids,
M K Sharief, and
R Hentges, and
E J Thompson
August 1986,
Journal of immunological methods,
M K Sharief, and
R Hentges, and
E J Thompson
November 1988,
Zhonghua nei ke za zhi,
M K Sharief, and
R Hentges, and
E J Thompson
October 1990,
Japanese journal of medical science & biology,
M K Sharief, and
R Hentges, and
E J Thompson
April 2019,
Psychiatry and clinical neurosciences,
M K Sharief, and
R Hentges, and
E J Thompson
July 1980,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
