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Complete HLA typing including HLA--A, --B, --C, --DR (D related B cell typing), --D, mixed lymphocyte culture (MLC), and primed lymphocyte testing (PLT), together with complete red blood cell (RBC), glyoxalase (GLO), GBG (Factor B), and phosphoglucomutase 3 (PGM3) typings were performed on a informative family. The five siblings inherited the four possible combinations of parental HLA haplotypes, and two of the siblings were HLA--A, --B, --C and --DR identical. Repeated MLC testing of the family revealed positive mixed lymphocyte reactivity in all combinations. B cell typing for the DR specificities demonstrated no variation from the expected inheritance pattern and specifically no recombination event. GBG and GLO typings militated against a recombination involving the paternal chromosome. HLA--D testing revealed that only one of the HLA--A, --B, --C, and --DR identical siblings gave typing responses to the HLA--Dw3 specificity present on that maternal haplotype. Utilizing HLA haploidentical combinations, lymphocytes were primed against the four parental haplotypes and the non-Dw3 haplotype of interest (Aw24--B8--DRw3--LDY) in the PLT. The sibling inheriting this haplo-type did not restimulate cells primed against the A2--B40--DRW6--LDY specificity. Furthermore, no discrimination was observed in the restimulation of lymphocytes primed against this haplo-type. Possible interpretations of these family data include: a spontaneous mutation, non-major histocompatibility locus (MHC) stimulation, and HLA--DR/D recombination.
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
February 1988,
Israel journal of medical sciences,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
February 1990,
Transplantation proceedings,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
January 1987,
Annals of clinical and laboratory science,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
August 1977,
Bratislavske lekarske listy,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
January 1973,
Surgical forum,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
September 1980,
European journal of immunology,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
October 1985,
Lancet (London, England),
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
May 1974,
Transplantation,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
October 1994,
Lymphokine and cytokine research,
N L Reinsmoen, and
H J Noreen, and
P S Friend, and
E R Giblett, and
L J Greenberg, and
J H Kersey
September 1973,
Transplantation,
