-transparent.png){kind=logo}
The presence of estrogen binding components (EBC) in intestinal mucosa of female rats was investigated by competitive-binding assay using radiolabelled and nonlabelled estradiol 17 beta (E2). EBC were found exclusively in the nuclear fraction and were absent from the cytosolic and from the microsomal fractions. Two types of nuclear EBC with different binding characteristics and capacities were found: Kd1 = 4.8 +/- 0.8 nM, n1 = 18.4 +/- 4.2 fmol/mg protein (n1 = 83.4 +/- 12.5 fmol/mg DNA) and Kd2 = 31.1 +/- 6.8 nM, n2 = 91.1 +/- 18.5 fmol/mg protein (412.7 +/- 80.0 fmol/mg DNA). Type 1 component showed slightly greater affinity for estrogens as compared to progesterone and dexamethasone whereas type 2 component bound other competitors with even greater affinity than E2.
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
August 1978,
Nihon eiseigaku zasshi. Japanese journal of hygiene,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
December 1973,
Nihon eiseigaku zasshi. Japanese journal of hygiene,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
January 1986,
Problemy endokrinologii,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
May 1971,
Endocrinology,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
January 1983,
Neurochemistry international,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
January 1974,
Neuroendocrinology,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
January 1978,
Fiziolohichnyi zhurnal,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
August 1978,
Brain research,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
October 1981,
Biochimica et biophysica acta,
H Lenasi, and
K Breskvar, and
T Hudnik-Plevnik
February 1974,
Brain research,
