Biomaterial Database

Structure of the Aspergillus nidulans qut repressor-encoding gene: implications for the regulation of transcription initiation. 1992

A R Hawkins, and H K Lamb, and C F Roberts

Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, U.K.

The nucleotide (nt) sequence of the qutR gene has been determined and shown to encode an inferred protein (QUTR) of 929 amino acids (aa). The inferred aa sequence shows a high level of similarity throughout its length with the aa sequence of the three C-terminal domains (shikimate kinase; 3-dehydroquinase; shikimate dehydrogenase) of the pentafunctional AROM protein of Aspergillus nidulans that catalyses steps 2-6 in the shikimate pathway. The inferred QUTR aa sequence has a completely conserved aa sequence motif, Gly, Xaa4, Gly, Lys, Ser, that is found in proteins that bind purine nt, suggesting that the inferred protein may have an in vivo kinase activity. The inferred QUTR protein also has a peptide sequence, DMVRLTQPAT, related to the active-site peptide in type-I 3-dehydroquinases. In active 3-dehydroquinases, the Arg (of QUTR) is replaced by Lys, which is involved in Schiff base formation as part of the reaction mechanism. The change from Lys----Arg in the inferred QUTR protein may allow the protein to bind but not metabolise the substrate for 3-dehydroquinase enzymes, namely 3-dehydroquinate. These observations are entirely consistent with the genetical model for how the QUTR protein functions, as it predicts that the protein can recognise and bind, but not metabolise, quinate, 3-dehydroquinate, and dehydroshikimate.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011801	Quinic Acid	An acid which is found in cinchona bark and elsewhere in plants. (From Stedman, 26th ed)	Quinate,Acid, Quinic
D012097	Repressor Proteins	Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.	Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D005075	Biological Evolution	The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.	Evolution, Biological
D005800	Genes, Fungal	The functional hereditary units of FUNGI.	Fungal Genes,Fungal Gene,Gene, Fungal
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001233	Aspergillus nidulans	A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.	Aspergillus nidulellus,Emericella nidulans
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D015966	Gene Expression Regulation, Fungal	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.	Fungal Gene Expression Regulation,Regulation of Gene Expression, Fungal,Regulation, Gene Expression, Fungal