Biomaterial Database

Effect of lead ions on rat erythrocyte purine content. 2004

Irena Baranowska-Bosiacka, and Alina Joanna Hlynczak

Department of Biochemistry, Faculty of Natural Sciences, University of Szczecin, Szczecin, Poland.

The influence of short-term exposure to lead on the energetic status of erythrocytes in rats is reported in this study. The male Wistar rats selected for this study drank water containing 1% lead(II) acetate and/or intraperitoneal injections of 1 or 2 mg/kg body wt every 4 d starting on the eighth of the experiment, over a period of 1 mo. The whole-blood lead concentration measured after 4 wk was 1.51-35.31 microg/dL. The concentrations of adenosine, adenosine triphosphates, diphosphates, and monophosphates (ATP, ADP, and AMP), guanine triphosphates, diphosphates and monophosphates (GTP, GDP, and GMP), guanosine (Guo), inosine (Ino), inosine monophosphate (IMP), hypoxantine (Hyp), and nicotinamide dinucleotide and its phosphate (NAD(+) and NADP(+)) were determined by high-performance liquid chromatography (HPLC). The mean concentrations of ATP, GTP, NAD(+), and NADP(+) and those of adenylate (AEC) and guanylate (GEC) were significantly reduced in erythrocytes from the animals exposed to lead when compared to untreated controls. These results suggest that a lead ion disrupts the erythrocyte energy pathways. The decreases of NAD(+) and ATP could be used as an indicator of the extent of exposure to low levels of lead.

UI	MeSH Term	Description	Entries
D007274	Injections, Intraperitoneal	Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall.	Intraperitoneal Injections,Injection, Intraperitoneal,Intraperitoneal Injection
D007292	Inosine Nucleotides		Inosine Phosphates,Nucleotides, Inosine,Phosphates, Inosine
D008297	Male		Males
D009243	NAD	A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)	Coenzyme I,DPN,Diphosphopyridine Nucleotide,Nadide,Nicotinamide-Adenine Dinucleotide,Dihydronicotinamide Adenine Dinucleotide,NADH,Adenine Dinucleotide, Dihydronicotinamide,Dinucleotide, Dihydronicotinamide Adenine,Dinucleotide, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide,Nucleotide, Diphosphopyridine
D009249	NADP	Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)	Coenzyme II,Nicotinamide-Adenine Dinucleotide Phosphate,Triphosphopyridine Nucleotide,NADPH,Dinucleotide Phosphate, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide Phosphate,Nucleotide, Triphosphopyridine,Phosphate, Nicotinamide-Adenine Dinucleotide
D009942	Organometallic Compounds	A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed)	Metallo-Organic Compound,Metallo-Organic Compounds,Metalloorganic Compound,Organometallic Compound,Metalloorganic Compounds,Compound, Metallo-Organic,Compound, Metalloorganic,Compound, Organometallic,Compounds, Metallo-Organic,Compounds, Metalloorganic,Compounds, Organometallic,Metallo Organic Compound,Metallo Organic Compounds
D011685	Purine Nucleotides	Purines attached to a RIBOSE and a phosphate that can polymerize to form DNA and RNA.	Nucleotides, Purine
D011687	Purines	A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
D004734	Energy Metabolism	The chemical reactions involved in the production and utilization of various forms of energy in cells.	Bioenergetics,Energy Expenditure,Bioenergetic,Energy Expenditures,Energy Metabolisms,Expenditure, Energy,Expenditures, Energy,Metabolism, Energy,Metabolisms, Energy
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle