BIOMAT Database Logo BIOMAT Database Logo

Beta3 integrins facilitate matrix interactions during transendothelial migration of PC3 prostate tumor cells. 2005

Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada.

BACKGROUND beta3 integrins play a role in metastatic progression of prostate cancer by mediating adhesion of cancer cells to endothelium and migration through extracellular matrix (ECM). However, the role of beta3 integrins during transendothelial migration (TEM) of prostate tumor cells is poorly understood. We examined the role of beta3 integrins in TEM of PC3 human prostate cancer cells through a monolayer of human lung microvascular endothelial cells (HLMVECs). METHODS PC3 cells were challenged with beta3 integrin antibodies or antisense nucleotides and their efficiency to migrate through monolayers of endothelial cells (ECs) was assessed using confocal microscopy. RESULTS beta3 integrins in PC3 cells are not localized in focal contacts and their blockade significantly inhibited TEM by over 50% preferentially during late stages of migration. Formation of PC3 cell pseudopodia on matrigel was significantly reduced by beta3 integrin antisense oligonucleotides. CONCLUSIONS beta3 integrins play important roles during TEM of PC3 cells while interacting with the matrix underneath the endothelium. These interactions are independent of the ability to cluster beta3 integrins into focal adhesions.

UI MeSH Term Description Entries
D007797 Laminin Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion. Merosin,Glycoprotein GP-2,Laminin M,Laminin M Chain,Chain, Laminin M,Glycoprotein GP 2,M Chain, Laminin
D008297 Male Males
D008833 Microcirculation The circulation of the BLOOD through the MICROVASCULAR NETWORK. Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D011471 Prostatic Neoplasms Tumors or cancer of the PROSTATE. Cancer of Prostate,Prostate Cancer,Cancer of the Prostate,Neoplasms, Prostate,Neoplasms, Prostatic,Prostate Neoplasms,Prostatic Cancer,Cancer, Prostate,Cancer, Prostatic,Cancers, Prostate,Cancers, Prostatic,Neoplasm, Prostate,Neoplasm, Prostatic,Prostate Cancers,Prostate Neoplasm,Prostatic Cancers,Prostatic Neoplasm
D011509 Proteoglycans Glycoproteins which have a very high polysaccharide content. Proteoglycan,Proteoglycan Type H
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004338 Drug Combinations Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture. Drug Combination,Combination, Drug,Combinations, Drug
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Cell-cell interactions during transendothelial migration of tumor cells.
November 1998, Microscopy research and technique,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Human neutrophils facilitate tumor cell transendothelial migration.
April 2001, American journal of physiology. Cell physiology,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Beta3-integrins rather than beta1-integrins dominate integrin-matrix interactions involved in postinjury smooth muscle cell migration.
May 1998, Circulation,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Integrins, cell matrix interactions and cell migration strategies: fundamental differences in leukocytes and tumor cells.
January 1998, Cell adhesion and communication,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Effects of thrombin on interactions between beta3-integrins and extracellular matrix in platelets and vascular cells.
November 2003, Arteriosclerosis, thrombosis, and vascular biology,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Tumor cell nuclei soften during transendothelial migration.
May 2021, Journal of biomechanics,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Breast tumor cells transendothelial migration induces endothelial cell anoikis through extracellular matrix degradation.
June 2009, Anticancer research,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
CXCR4 chemokine receptor mediates prostate tumor cell adhesion through alpha5 and beta3 integrins.
April 2006, Neoplasia (New York, N.Y.),
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Melatonin receptors in PC3 human prostate tumor cells.
May 1999, Journal of pineal research,
Xiaowei Wang, and Alexander M Ferreira, and Qing Shao, and Dale W Laird, and Martin Sandig
Rac1 activation driven by 14-3-3ΞΆ dimerization promotes prostate cancer cell-matrix interactions, motility and transendothelial migration.
January 2012, PloS one,
Copied contents to your clipboard!