-transparent.png){kind=logo}
Alpha2,3-sialylation of the lactosamine type N-glycans with trans-sialidase from Trypanosoma cruzi is reported. Trans-sialidase (160 kDa, pI 5.35-5.65) and its catalytic fragment (70 kDa, pI 6.0-6.3) were isolated from T. cruzi cells and immobilized on ConA-Sepharose. The resulting preparation retained activity for several months and was repeatedly used for obtaining mono-, di-, tri-, and tetrasialylated 7-amino-4-metylcoumarine-labeled oligosaccharides with various numbers of antennas and for alpha2,3-sialylation of glycans within glycoproteins and neoglycoconjugates.
Related Publications
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
July 2006,
Cellular microbiology,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
July 2006,
Carbohydrate research,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
April 1993,
European journal of biochemistry,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
April 2005,
Journal of immunology (Baltimore, Md. : 1950),
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
August 1999,
Biochemical Society transactions,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
June 1993,
Parasitology today (Personal ed.),
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
December 1992,
Glycobiology,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
May 1996,
Molecular and biochemical parasitology,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
January 1993,
Annual review of microbiology,
S D Shiian, and
V S Zueva, and
V V Nasonov, and
L S Zhigis, and
N Ts Tsoĭ, and
N B Bovin
January 2008,
Cellular microbiology,
