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We report a rather simple method to determine glucose concentration in serum, using isotope dilution mass spectrometry and [13C6]glucose as internal standard. The procedure involves a single step of sample purification and the conversion of the analyte into its aldononitrile pentaacetate. The between-day and within-day contribution to total variance for a single measurement was determined by assaying Standard Reference Material (SRM) 909 serum. The method was then applied to measurement of glucose concentration in three lyophilized sera: SRM 909 and two other commercially available sera. In the two studies, the concentration of SRM 909 serum was found to be 0.8% above and 0.3% below the reported value (6.25 mmol/L), respectively; the overall coefficient of variation for determinations in all sera ranged from 0.37% to 0.56%. The precision and the accuracy of the method satisfy the requirements for a Definitive Method.
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
July 1986,
Analytical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
April 1984,
Analytical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
June 1980,
Clinical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
October 1990,
Analytical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
May 1984,
Clinical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
October 2016,
Analytical and bioanalytical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
March 1995,
Clinical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
June 1982,
Clinical chemistry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
September 1982,
Biomedical mass spectrometry,
F Magni, and
R Paroni, and
P A Bonini, and
M G Kienle
November 2004,
Analytical and bioanalytical chemistry,
