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The study evaluated whether a vaccine can be designed by using dendritic cells loaded by mycobacterial antigens. A method for preparing murine dendritic cells from the bone-marrow precursors was modified. The dendritic cells were characterized by the expression of surface molecules by flow cytofluorometry. The study has shown that T lymphocytes can be primed in vivo by administering the dendritic cells expressing the protective Mycobacterium tuberculosis antigens H37Rv.
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